Flow cytometric identification of murine neutrophils and monocytes

被引：297

作者：

Lagasse, E

Weissman, IL

机构：

[1] Dept. of Pathol. and Devmtl. Biology, Stanford Univ. School of Medicine, Stanford

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1996年 / 197卷 / 1-2期

关键词：

monocyte; neutrophil; flow cytometry; Gr-1; Mac-1;

D O I：

10.1016/0022-1759(96)00138-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Flow cytometry and cell sorting have been employed in order to identify and purify murine neutrophils and monocytes. Using a combination of antibodies, we were able to distinguish between these two subsets of myeloid cells. The method described in this paper is simple to perform and can be applied to characterize myeloid cells from blood, spleen, bone marrow acid after an induced inflammation.

引用

页码：139 / 150

页数：12

共 21 条

[1] SURFACE-ANTIGEN EXPRESSION AND IMMUNOGLOBULIN GENE REARRANGEMENT DURING MOUSE PRE-B-CELL DEVELOPMENT [J].