Definition of the gene encoding the minor histocompatibility antigen HA-1 and typing for HA-1 from genomic DNA

被引:27
作者
Tseng, LH
Lin, MT
Martin, PJ
Pei, J
Smith, AG
Hansen, JA
机构
[1] Fred Hutchinson Canc Res Ctr, Div Clin Res, Human Immunogenet Program, Seattle, WA 98109 USA
[2] Natl Taiwan Univ Hosp, Dept Med Genet & Oncol, Taipei, Taiwan
[3] Washington Univ, Dept Internal Med, Seattle, WA USA
来源
TISSUE ANTIGENS | 1998年 / 52卷 / 04期
关键词
HA-1; gene; inverse PCR; marrow transplantation; minor histocompatibility antigen;
D O I
10.1111/j.1399-0039.1998.tb03052.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Recipient mismatching for the minor histocompatibility antigen HA-1 has been associated with acute graft-Versus-host disease after allogeneic marrow transplantation. Two polymorphic nucleotides near an exon-intron junction of the gene encoding this minor histocompatibility antigen have been identified. In this study we determined the genomic DNA sequence of the intron downstream from this polymorphic exon. Based on this sequence, primers were designed to amplify the genomic KA-I gene sequence, and analysis of restriction fragment length polymorphisms was used to assign the HA-1 genotypes of 160 unrelated probands and a paired sibling for each proband. Among probands, the HA-1(H) allele frequency was 0.441, and the HA-1(R) allele frequency was 0.559, The distribution of HA-1 genotypes showed close fit with Hardy-Weinberg equilibrium. Likewise, die number of sibling pairs with disparity for KA-I alleles showed close fit with predictions based on Hardy-Weinberg equilibrium. These results provide a simple and well Validated method for future studies correlating HA-1 disparity with clinical outcome after allogeneic marrow transplantation.
引用
收藏
页码:305 / 311
页数:7
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