Isolation and characterization of twenty Microsatellite loci in Japanese sea cucumber (Stichopus japonicus)

被引：57

作者：

Kanno, M

Li, Q

Kijima, A ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Agr Sci, Integrated Ctr Field Sci, Onagawa, Miyagi 9862242, Japan

[2] Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China

来源：

MARINE BIOTECHNOLOGY | 2005年 / 7卷 / 03期

关键词：

sea cucumber; Stichopus japonicus; microsatellite; locigenetic variability;

D O I：

10.1007/s10126-004-0006-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Twenty microsatellite markers were first developed from the Japanese sea cucumber Stichopus japonicus using an enrichment protocol. Of the 20 microsatellite loci, 19 loci were polymorphic in the population examined. At these polymorphic loci, the number of alleles per locus varied from 2 to 15, and the observed heterozygosities ranged from 0.03 to 0.97, which is considerably higher than those previously found for allozymes. The high variability of the microsatellite markers identified in this study will make them excellent tools for genetic analyses of S. japonicus.

引用

页码：179 / 183

页数：5

共 20 条

[1] Complex evolution of a salmonid microsatellite locus and its consequences in inferring allelic divergence from size information
Angers, B
Bernatchez, L
[J]. MOLECULAR BIOLOGY AND EVOLUTION, 1997, 14 (03) : 230 - 238
[2] [Anonymous], 1995, GUIDE SEASHORE ANIMA
[3] Bierne N, 1998, GENETICS, V148, P1893
[4] CALLEN DF, 1993, AM J HUM GENET, V52, P922
[5] Rapid isolation of CA microsatellites from the tilapia genome
Carleton, KL
Streelman, JT
Lee, BY
Garnhart, N
Kidd, M
Kocher, TD
[J]. ANIMAL GENETICS, 2002, 33 (02) : 140 - 144
[6] CHOE S, 1961, NIPPON SUISAN GAKK, V27, P97
[7] Microsatellites in the sand lizard (Lacerta agilis):: Description, variation, inheritance, and applicability
Gullberg, A
Tegelström, H
Olsson, M
[J]. BIOCHEMICAL GENETICS, 1997, 35 (7-8) : 281 - 295
[8] The molecular basis of a microsatellite null allele from the white sands pupfish
Jones, AG
Stockwell, CA
Walker, D
Avise, JC
[J]. JOURNAL OF HEREDITY, 1998, 89 (04) : 339 - 342
[9] Genetic differentiation among three color variants of Japanese sea cucumber Stichopus japonicus
Kan-No, M
Kijima, A
[J]. FISHERIES SCIENCE, 2003, 69 (04) : 806 - 812
[10] Kan-no Manami, 2002, Suisan Zoshoku, V50, P63

← 1 2 →