A one-step method to construct a third-generation biosensor based on horseradish peroxidase and gold nanoparticles embedded in silica sol-gel network on gold modified electrode

被引：60

作者：

Di, JW ^{[1
]}

Shen, CP ^{[1
]}

Peng, SH ^{[1
]}

Tu, YF ^{[1
]}

Li, SJ ^{[1
]}

机构：

[1] Suzhou Univ, Dept Chem, Suzhou 215006, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2005年 / 553卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

horseradish peroxidase; direct electron transfer; gold nanoparticles; silica sol-gel; biosensor;

D O I：

10.1016/j.aca.2005.08.013

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A one-step method for fabrication of horseradish peroxidase (HRP) biosensor has been developed. The gold nanoparticles and HRP were simultaneously embedded in silica sol-gel network on gold electrode surface in the presence of cysteine. The immobilized HRP exhibited direct electrochemical behavior toward the reduction of hydrogen peroxide. The heterogeneous electron transfer rate constant was evaluated to be 7.8 s(-1). The biosensor displayed an excellent elctrocatalytic response to the reduction of H2O2 without any mediator. The calibration range of H2O2 was from 1.6 mu mol L-1 to 3.2 mmol L-1 and a detection limit of 0.5 mu mol L-1 at a signal-to-noise ratio of 3. The K-M(app) value of HRP immobilized on the electrode surface was found to be 1.1 mmol L-1. The biosensor exhibited high sensitivity, rapid response and long-term stability. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：196 / 200

页数：5

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