Reverse transcriptase-like activity in Trypanosoma cruzi

被引：7

作者：

Gonzalez, CI

Thomas, MC

Martin, F

Alcami, J

Alonso, C

Lopez, MC

机构：

[1] CSIC,INST PARASITOL & BIOMED LOPEZ NEGRA,DEPT MOL BIOL,GRANADA 18001,SPAIN

[2] HOSP 12 OCTUBRE,CTR INVEST,E-28041 MADRID,SPAIN

[3] UNIV AUTONOMA MADRID,CSIC,CTR BIOL MOL,E-28049 MADRID,SPAIN

来源：

ACTA TROPICA | 1997年 / 63卷 / 2-3期

关键词：

Trypanosoma cruzi; RNA dependent polymerase; RNA homopolymers; RT-like inhibitors;

D O I：

10.1016/S0001-706X(96)00611-0

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The use of specific synthetic RNA homopolymers as templates and short oligonucleotides as primers has allowed evidence of the existence of a reverse transcriptase-like activity in Trypanosoma cruzi, to be revealed. The RNA:DNA products derived from this reaction are of approximately 110 nucleotides in length. The enzyme has greater affinity for poly(rA)/ oligo(dT) templates than for poly(rC)/oligo(dG) having a 20 mM Mg+ (+) ion requirement. The detected reverse transcriptase-like activity is not affected by aphidicolin and ddTTP but is inhibited by actinomycin D, novobiocin, rifamycin SV and AZT. (C) 1997 Elsevier Science B.V. All rights reserved.

引用

页码：117 / 126

页数：10

共 21 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

BROWN TA, 1991, MOL BIOL, V5, P139

[3] DETECTION OF ENTEROVIRUSES IN CELL-CULTURES BY USING INSITU TRANSCRIPTION [J].