Growth and membrane polarization in Pseudomonas aeruginosa UG2 grown in randomized microgravity in a high aspect ratio vessel

被引：37

作者：

England, LS ^{[1
]}

Gorzelak, M ^{[1
]}

Trevors, JT ^{[1
]}

机构：

[1] Univ Guelph, Dept Environm Biol, Lab Microbial Technol, Guelph, ON N1G 2W1, Canada

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2003年 / 1624卷 / 1-3期

基金：

加拿大自然科学与工程研究理事会;

关键词：

bacteria; fluorescent probe; growth; membrane fluidity; membrane polarization; Pseudomonas aeruginosa; randomized microgravity (RMG);

D O I：

10.1016/j.bbagen.2003.09.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Growth and membrane polarization of Pseudomonas aeruginosa UG2 cells grown under randomized microgravity (RMG) and 1 x g were measured in a high aspect ratio vessel (HARV) and also in batch cultures mixed at 12 and 150 rpm in Erlenmeyer shake flasks. Membrane polarization was measured using the fluorescent probe, 1,6-diphenyl-1,3,5-hexatriene (DPH). No differences were observed in the growth curves or membrane polarization values (about 0.300) under all three culture conditions. However, the net effect of RMG at the single cell level may be still unknown. It may be possible that RMG effects are species-dependent or bacterial cells with a small mass and volume may be near the threshold where RMG exerts a minimal effect. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：76 / 80

页数：5

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