Iloprost suppresses connective tissue growth factor production in fibroblasts and in the skin of scleroderma patients

被引:172
作者
Stratton, R
Xu, SW
Martini, G
Holmes, A
Leask, A
Haberberger, T
Martin, GR
Black, CM
Abraham, D
机构
[1] UCL, Royal Free & Univ Coll Med Sch, Ctr Rheumatol, London NW3 2PF, England
[2] FibroGen Inc, San Francisco, CA USA
关键词
D O I
10.1172/JCI12020
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Patients with scleroderma receiving Iloprost as a treatment for severe Raynaud's phenomenon report a reduction in skin tightness, suggesting that this drug inhibits skin fibrosis, Connective tissue growth factor (CTGF), a recently described profibrotic cytokine, acts downstream and in concert with TGF-beta to stimulate the fibrotic process and is involved in the fibrosis seen in scleroderma. Here we show that Iloprost, acting by elevation of cAMP, blocks the induction of CTGF and the increase in collagen synthesis in fibroblasts exposed to TGF-beta. The potency of Iloprost with respect to suppression of CTGF Far exceeds that of other prostanoid receptor agonists, suggesting that its effect is mediated by the prostacyclin receptor IF. By sampling dermal interstitial fluid using a suction blister device, we show that CTGF levels are greatly elevated in the dermis of scleroderma patients compared with healthy controls and that Iloprost infusion causes a marked decrease in dermal CTGF levels. These studies suggest that Iloprost could be reducing the level of a key profibrotic cytokine in scleroderma patients and that endogenous production of eicosanoids may limit the fibrotic response to TGF-beta.
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页码:241 / 250
页数:10
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