A potential strategy for high-grade gliomas: combination treatment with lithium chloride and BmK CT

被引：15

作者：

Fu, Yuejun ^{[1
]}

Zheng, Shuhua ^{[1
]}

Huang, Rui ^{[1
]}

An, Na ^{[1
]}

Zheng, Yali ^{[1
]}

Zhang, Zhiyun ^{[1
]}

Liang, Aihua ^{[1
]}

机构：

[1] Shanxi Univ, Inst Biotechnol, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2012年 / 34卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Buthus martensii Karsch CT; beta-Catenin; Glioma; Lithium chloride; Matrix metalloproteinase-2; GLYCOGEN-SYNTHASE KINASE-3; CELL-MIGRATION; MATRIX METALLOPROTEINASE-2; POTASSIUM CHANNELS; IN-VITRO; INVASION; EXPRESSION; DEATH;

D O I：

10.1007/s10529-011-0741-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Therapies for high-grade gliomas (HHG) that have strong tendency of infiltration and resistance to chemotherapies are currently unavailable. Here, we report that lower-dose combination therapy of Buthus martensii Karsch (BmK) CT, a type of scorpion toxin peptide, and LiCl, clinically used as mood stabilizer, could synergistically inhibit the migration, invasion and proliferation of C6 glioma cells. The decreased invasiveness of C6 glioma cells was accompanied by inhibited activation, catalytic activity and/or expression of matrix metalloproteinase-2. Moreover, TOPfalsh luciferase reporter and immunofluorescence staining showed altered localization pattern of beta-catenin at the leading edge of 2D scratch. Our results suggested that the combination treatment of lithium and BmK CT may constitute a novel and potential strategy for HHG therapy.

引用

页码：9 / 17

页数：9

共 26 条

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