Novel motility mutants of Synechocystis strain PCC 6803 generated by in vitro transposon mutagenesis

被引：52

作者：

Bhaya, D

Takahashi, A

Shahi, P

Grossman, AR

机构：

[1] Carnegie Inst Washington, Dept Plant Biol, Stanford, CA 94305 USA

[2] Riken Plant Sci Ctr, Lab Metab Compartmentat, Wako, Saitama 3510198, Japan

[3] San Jose State Univ, Dept Biol Sci, San Jose, CA 95192 USA

来源：

JOURNAL OF BACTERIOLOGY | 2001年 / 183卷 / 20期

关键词：

D O I：

10.1128/JB.183.20.6140-6143.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We screened for transposon-generated mutants of Synechocystis sp. strain PCC 6803 that exhibited aberrant phototactic movement. Of the 300 mutants generated, about 50 have been partially characterized; several contained transposons in genes encoding chernotaxis-related proteins, while others mapped to novel genes. These novel genes and their possible roles in motility are discussed.

引用

收藏

页码：6140 / 6143

页数：4

相关论文

共 17 条

[1]

Bateman A, 2004, NUCLEIC ACIDS RES, V32, pD138, DOI [10.1093/nar/gkp985, 10.1093/nar/gkh121, 10.1093/nar/gkr1065]

[2] Type IV pilus biogenesis and motility in the cyanobacterium Synechocystis sp PCC6803 [J].

Bhaya, D ;

Bianco, NR ;

Bryant, D ;

Grossman, A .

MOLECULAR MICROBIOLOGY, 2000, 37 (04) :941-951

[3] The role of an alternative sigma factor in motility and pilus formation in the cyanobacterium Synechocystis sp. strain PCC6803 [J].

Bhaya, D ;

Watanabe, N ;

Ogawa, T ;

Grossman, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (06) :3188-3193

[4] Light regulation of type IV pilus-dependent motility by chemosensor-like elements in Synechocystis PCC6803 [J].

Bhaya, D ;

Takahashi, A ;

Grossman, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (13) :7540-7545

[5]

Blatch GL, 1999, BIOESSAYS, V21, P932, DOI 10.1002/(SICI)1521-1878(199911)21:11<932::AID-BIES5>3.3.CO

[6]

2-E

[7] Coiled coils: a highly versatile protein folding motif [J].

Burkhard, P ;

Stetefeld, J ;

Strelkov, SV .

TRENDS IN CELL BIOLOGY, 2001, 11 (02) :82-88

[8] Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: the dense alignment surface method [J].

Cserzo, M ;

Wallin, E ;

Simon, I ;

vonHeijne, G ;

Elofsson, A .

PROTEIN ENGINEERING, 1997, 10 (06) :673-676

[9] A eukaryotic-type protein kinase, SpkA, is required for normal motility of the unicellular cyanobacterium Synechocystis sp strain PCC 6803 [J].

Kamei, A ;

Yuasa, T ;

Orikawa, K ;

Geng, XX ;

Ikeuchi, M .

JOURNAL OF BACTERIOLOGY, 2001, 183 (05) :1505-1510

[10] PREDICTING COILED COILS FROM PROTEIN SEQUENCES [J].

LUPAS, A ;

VANDYKE, M ;

STOCK, J .

SCIENCE, 1991, 252 (5009) :1162-1164