Limited performance of DNA barcoding in a diverse community of tropical butterflies

被引:305
作者
Elias, Marianne
Hill, Ryan I.
Willmott, Keith R.
Dasmahapatra, Kanchon K.
Brower, Andrew V. Z.
Malllet, James
Jiggins, Chris D.
机构
[1] Univ Edinburgh, Inst Evolutionary Biol, Edinburgh EH9 3JT, Midlothian, Scotland
[2] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA
[3] Univ Florida, Florida Museum Nat Hist, McGuire Ctr Lepidopetra & Biodivers, Gainesville, FL 32611 USA
[4] UCL, Dept Biol, London NW1 2HE, England
[5] Middle Tennessee State Univ, Dept Biol, Murfreesboro, TN 37132 USA
[6] Univ Cambridge, Dept Zool, Cambridge CB2 3EJ, England
基金
英国自然环境研究理事会;
关键词
DNA barcoding; Amazon; biodiversity; Lepidoptera; mimicry;
D O I
10.1098/rspb.2007.1035
中图分类号
Q [生物科学];
学科分类号
07 [理学]; 0710 [生物学]; 09 [农学];
摘要
DNA 'barcoding' relies on a short fragment of mitochondrial DNA to infer identification of specimens. The method depends on genetic diversity being markedly lower within than between species. Closely related species are most likely to share genetic variation in communities where speciation rates are rapid and effective population sizes are large, such that coalescence times are long. We assessed the applicability of DNA barcoding (here the 5' half of the cytochrome c oxidase 1) to a diverse community of butterflies from the upper Amazon, using a group with a well-established morphological taxonomy to serve as a reference. Only 77% of species could be accurately identified using the barcode data, a figure that dropped to 68% in species represented in the analyses by more than one geographical race and at least one congener. The use of additional mitochondrial sequence data hardly improved species identification, while a fragment of a nuclear gene resolved issues in some of the problematic species. We acknowledge the utility of barcodes when morphological characters are ambiguous or unknown, but we also recommend the addition of nuclear sequence data, and caution that species-level identification rates might be lower in the most diverse habitats of our planet.
引用
收藏
页码:2881 / 2889
页数:9
相关论文
共 70 条
[1]
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]
Phylogenetic discordance at the species boundary:: Comparative gene genealogies among rapidly radiating Heliconius butterflies [J].
Beltrán, M ;
Jiggins, CD ;
Bull, V ;
Linares, M ;
Mallet, J ;
McMillan, WO ;
Bermingham, E .
MOLECULAR BIOLOGY AND EVOLUTION, 2002, 19 (12) :2176-2190
[3]
Molecular systematics - Counting angels with DNA [J].
Blaxter, M .
NATURE, 2003, 421 (6919) :122-124
[5]
PHYLOGENY OF HELICONIUS BUTTERFLIES INFERRED FROM MITOCHONDRIAL-DNA SEQUENCES (LEPIDOPTERA, NYMPHALIDAE) [J].
BROWER, AV .
MOLECULAR PHYLOGENETICS AND EVOLUTION, 1994, 3 (02) :159-174
[6]
Phylogenetic relationships among the Ithomiini (Lepidoptera: Nymphalidae) inferred from one mitochondrial and two nuclear gene regions [J].
Brower, AVZ ;
Freitas, AVL ;
Lee, MM ;
Silva-Brandao, KL ;
Whinnett, A ;
Willmott, KR .
SYSTEMATIC ENTOMOLOGY, 2006, 31 (02) :288-301
[7]
Brower AVZ, 1996, ZOOL J LINN SOC-LOND, V116, P317
[8]
Gene trees, species trees, and systematics: A cladistic perspective [J].
Brower, AVZ ;
DeSalle, R ;
Vogler, A .
ANNUAL REVIEW OF ECOLOGY AND SYSTEMATICS, 1996, 27 :423-450
[10]
Who will actually use DNA barcoding and what will it cost? [J].
Cameron, Stephen ;
Rubinoff, Daniel ;
Will, Kipling .
SYSTEMATIC BIOLOGY, 2006, 55 (05) :844-847