An enzyme-immunoassay for the detection of the mycotoxin zearalenone by use of yolk antibodies

被引：17

作者：

Pichler, H

Krska, R

Szekacs, A

Grasserbauer, M

机构：

[1] IFA Tulln, Inst Agrobiotechnol, Ctr Analyt Chem, A-3430 Tulln, Austria

[2] Hungarian Acad Sci, Inst Plant Protect, H-1525 Budapest, Hungary

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1998年 / 362卷 / 01期

关键词：

D O I：

10.1007/s002160051053

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The development of an enzyme-immunoassay (ELISA) for the detection of the Fusarium mycotoxin zearalenone (ZON) is described. In contrast to the common antibody isolation from mammal serum, chicken were immunized in order to isolate specific antibodies from the egg yolk. Five weeks after the start of the immunization a titer of 1:76 000 resulted from three injections without any adverse effects for the animals. Utilizing the indirect competitive assay format ZON could be detected in a concentration range between 10 and 200 mu g/L.

引用

页码：176 / 177

页数：2

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