共 27 条
Electrochemical grafting by reduction of 4-aminoethylbenzenediazonium salt:: Application to the immobilization of (bio)molecules
被引:93
作者:
Griveau, Sophie
[1
,2
]
Mercier, Dimitri
[2
]
Vautrin-Ul, Christine
[2
]
Chausse, Annie
[2
]
机构:
[1] Univ Paris 05, Ecole Natl Super Chim Paris, INSERM,Fac Pharm,Chem & Genet Pharmacol Lab,U640, CNRS,UMR 8151, Paris, France
[2] Univ Evry Val Essonne, CNRS, CEA, Lab Anal Modelisat Biol & Environm,UMR 8587, F-91025 Evry, France
关键词:
electrochemical surface modification;
diazonium salts;
gold electrode;
glassy carbon electrode;
D O I:
10.1016/j.elecom.2007.09.004
中图分类号:
O646 [电化学、电解、磁化学];
学科分类号:
081704 ;
摘要:
We propose in this study a simple and rapid way to produce stable amino-derivatized conductive surfaces for the subsequent immobilization of (bio)molecules. This was achieved through the use of (4-aminoethyl)benzenediazonium salt (AEBD), which was immobilized on glassy carbon and gold electrodes by its electrochemical reduction. The presence of terminal grafted amino functions was evidenced with XPS by analyzing N1s core level. Besides this conventional surface characterisation, an electrochemical strategy is proposed here to evidence the presence of immobilized amines, in which the chemical reactivity of amines towards 2,4,6-trinitrobenzenesulfonic acid (TNBS) is used. Surface-bound TNBS served as an electrochemical marker and was detected by cyclic voltammetry. Additionally, pre-modified gold electrodes with amino functions can be derivatized with biomolecules such as glutathione (GSH). Glutathione attachment was evidenced by studying the electrochemical behaviour of ferri/ferrocyanide redox before and after its immobilization. The functionalized electrodes were then used for the detection of copper ions in neutral aqueous solutions. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:2768 / 2773
页数:6
相关论文