Solution structure of a protein inhibitor of neuronal nitric oxide synthase

被引:44
作者
Tochio, H [1 ]
Ohki, S [1 ]
Zhang, Q [1 ]
Li, M [1 ]
Zhang, MJ [1 ]
机构
[1] Hong Kong Univ Sci & Technol, Dept Biochem, Hong Kong, Peoples R China
关键词
D O I
10.1038/2940
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The structure of the neuronal nitric oxide synthase inhibitory protein, PIN (protein inhibitor of nNOS), has been determined by NMR spectroscopy. Two N-terminal antiparallel alpha-helices pack against a four-stranded antiparallel beta-sheet in the C-terminal region of the protein, forming a two-layer alpha/beta plait. The three dimensional structure of PIN resembles the fold of the B-chain of spartylglucosaminidase. A non-prolyl cis peptide bond was found between Pro 52 and Thr 53 of the protein. PIN has a large solvent-exposed hydrophobic surface that contains a cavity and is rimmed with positive charges. This surface may serve as the primary target-binding region for this multi-functional regulatory protein.
引用
收藏
页码:965 / 969
页数:5
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