Characterization of a membrane-bound arginine-specific serine protease from rat intestinal mucosa

被引：10

作者：

Kishi, K

Yamazaki, K

Yasuda, I

Yahagi, N

Ichinose, M

Tsuchiya, Y

Athauda, SBP

Inoue, H

Takahashi, K ^{[1
]}

机构：

[1] Toho Univ, Sch Med, Dept Biochem, Tokyo 1438540, Japan

[2] Tokyo Univ Pharm & Life Sci, Sch Life Sci, Hachioji, Tokyo 1920392, Japan

[3] Univ Tokyo, Fac Med, Dept Internal Med, Tokyo 1138655, Japan

[4] Univ Peradeniya, Fac Med, Dept Biochem, Peradeniya, Sri Lanka

来源：

JOURNAL OF BIOCHEMISTRY | 2001年 / 130卷 / 03期

关键词：

brushborder membrane; intestinal mucosa; serine protease; subcellular localization; substrate specificity;

D O I：

10.1093/oxfordjournals.jbchem.a003002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previously we isolated and characterized a membrane-bound, arginine-specific serine protease from pig intestinal mucosa [J. Biol Chem. 269, 32985-32991 (1994)]. For further characterization of this type of enzyme, we cloned a cDNA from rat intestinal mucosa encoding the precursor of a similar protease. The partial amino acid sequences determined for the pig enzyme were found to be shared almost completely by the rat enzyme. The serine protease domain of the rat enzyme, heterologously expressed in Escherichia coli, specifically cleaved Arg (or Lys)-X bonds with a marked preference for Arg-Arg or Arg-Lys, similar to the pig enzyme. The mRNA for the rat enzyme was shown to be distributed mainly in intestine, and the enzyme was detected in the duodenal mucosa as a 70 kDa protein. Immunohistochemical analysis of the small intestinal tissue showed that the enzyme is localized mainly on brushborder membranes.

引用

页码：425 / 430

页数：6

共 13 条

[1] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].