Sandwich ELISA detection of Clostridium perfringens cells and α-toxin from field cases of necrotic enteritis of poultry

被引：25

作者：

McCourt, MT

Finlay, DA

Laird, C

Smyth, JA

Bell, C

Ball, HJ

机构：

[1] Dept Agr & Rural Dev No Ireland, Vet Sci Div, Belfast BT4 3SD, Antrim, North Ireland

[2] Queens Univ Belfast, Dept Vet Sci, Belfast BT4 3SD, Antrim, North Ireland

来源：

VETERINARY MICROBIOLOGY | 2005年 / 106卷 / 3-4期

关键词：

necrotic enteritis; Clostridium perfringens; sandwich ELISA; alpha-toxin;

D O I：

10.1016/j.vetmic.2004.12.023

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sandwich ELISAs (sELISAs) for the detection of Clostridium perfringens cells and a-toxin were developed and used to screen intestinal samples from normal broiler chickens and from clinical cases of necrotic enteritis. The assays clearly distinguished between the two sets of samples. The sELISA absorbance values from samples obtained from the majority of healthy birds were low and those from the majority of necrotic enteritis cases were high. Together, the assays provide a suitable test for the rapid screening for the diagnosis of necrotic enteritis in poultry. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：259 / 264

页数：6

共 14 条

[1] INTERACTION OF CLOSTRIDIUM-PERFRINGENS AND ITS TOXINS IN PRODUCTION OF NECROTIC ENTERITIS OF CHICKENS [J].