High-resolution physical mapping by combined Alu-hybridization/PCR screening: Construction of a yeast artificial chromosome map covering 31 centimorgans in 3p21-p14

被引：11

作者：

Aburatani, H ^{[1
]}

Stanton, VP ^{[1
]}

Housman, DE ^{[1
]}

机构：

[1] MIT,CTR CANC RES,CAMBRIDGE,MA 02139

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 09期

关键词：

Alu-PCR; chromosome; 3;

D O I：

10.1073/pnas.93.9.4474

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We describe an integrated approach to large-scale physical mapping using an Alu-PCR hybridization screening strategy in conjunction with direct PCR-based screening to construct a continuous yeast artificial chromosome map covering >20 mb in human chromosome 3, bands p14-p21, composed of 205 loci, connected by 480 yeast artificial chromosomes, with average interlocus distance of approximate to 100 kb. We observe an inverse distribution of Alu-PCR and (CA), markers. These results suggest that the two screening methods may be complementary and demonstrate the utility of Alu-PCR hybridization screening in the closure of high-resolution human physical maps.

引用

页码：4474 / 4479

页数：6

共 26 条

[1] CONSTRUCTION AND CHARACTERIZATION OF A YEAST ARTIFICIAL CHROMOSOME LIBRARY CONTAINING 7 HAPLOID HUMAN GENOME EQUIVALENTS
ALBERTSEN, HM
ABDERRAHIM, H
CANN, HM
DAUSSET, J
LEPASLIER, D
COHEN, D
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (11) : 4256 - 4260
[2] ATLSCHUL SF, 1990, J MOL BIOL, V215, P403
[3] BELL CJ, 1995, HUM MOL GENET, V4, P59
[4] MAPPING THE WHOLE HUMAN GENOME BY FINGERPRINTING YEAST ARTIFICIAL CHROMOSOMES
BELLANNECHANTELOT, C
LACROIX, B
OUGEN, P
BILLAULT, A
BEAUFILS, S
BERTRAND, S
GEORGES, I
GLIBERT, F
GROS, I
LUCOTTE, G
SUSINI, L
CODANI, JJ
GESNOUIN, P
POOK, S
VAYSSEIX, G
LUKUO, J
RIED, T
WARD, D
CHUMAKOV, I
LEPASLIER, D
BARILLOT, E
COHEN, D
[J]. CELL, 1992, 70 (06) : 1059 - 1068
[5] EXON AMPLIFICATION - A STRATEGY TO ISOLATE MAMMALIAN GENES BASED ON RNA SPLICING
BUCKLER, AJ
CHANG, DD
GRAW, SL
BROOK, JD
HABER, DA
SHARP, PA
HOUSMAN, DE
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (09) : 4005 - 4009
[6] CONTINUUM OF OVERLAPPING CLONES SPANNING THE ENTIRE HUMAN CHROMOSOME-21Q
CHUMAKOV, I
RIGAULT, P
GUILLOU, S
OUGEN, P
BILLAUT, A
GUASCONI, G
GERVY, P
LEGALL, I
SOULARUE, P
GRINAS, L
BOUGUELERET, L
BELLANNECHANTELOT, C
LACROIX, B
BARILLOT, E
GESNOUIN, P
POOK, S
VAYSSEIX, G
FRELAT, G
SCHMITZ, A
SAMBUCY, JL
BOSCH, A
ESTIVILL, X
WEISSENBACH, J
VIGNAL, A
RIETHMAN, H
COX, D
PATTERSON, D
GARDINER, K
HATTORI, M
SAKAKI, Y
ICHIKAWA, H
OHKI, M
LEPASLIER, D
HEILIG, R
ANTONARAKIS, S
COHEN, D
[J]. NATURE, 1992, 359 (6394) : 380 - 387
[7] MOLECULAR-CLONING OF THE HUMAN PROTOONCOGENE WNT-5A AND MAPPING OF THE GENE (WNT5A) TO CHROMOSOME 3P14-P21
CLARK, CC
COHEN, I
EICHSTETTER, I
CANNIZZARO, LA
MCPHERSON, JD
WASMUTH, JJ
IOZZO, RV
[J]. GENOMICS, 1993, 18 (02) : 249 - 260
[8] A 1ST-GENERATION PHYSICAL MAP OF THE HUMAN GENOME
COHEN, D
CHUMAKOV, I
WEISSENBACH, J
[J]. NATURE, 1993, 366 (6456) : 698 - 701
[9] Dausset J, 1992, Behring Inst Mitt, P13
[10] DOBROVIC A, 1988, CANCER RES, V48, P682

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