Pituitary lactotrope expresses transforming growth factor beta (TGF beta) type II receptor mRNA and protein and contains I-125-TGF beta 1 binding sites
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De, A
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机构:WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164
De, A
Morgan, TE
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机构:WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164
Morgan, TE
Speth, RC
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机构:WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164
Speth, RC
Boyadjieva, N
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机构:WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164
Boyadjieva, N
Sarkar, DK
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机构:WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164
Sarkar, DK
机构:
[1] WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164
[2] UNIV SO CALIF,DEPT BIOL SCI,ETHEL PERCY ANDRUS GERONTOL CTR,LOS ANGELES,CA 90089
Transforming growth factor beta 1 (TGF beta 1) has recently been shown to be produced in the prolactin (PRL)-secreting lactotropes of the pituitary gland. TGF beta 1 inhibits lactotropic secretion and proliferation, and the production of TGF beta 1 in lactotropes is reduced during lactotropic growth following estrogen treatment in ovariectomized rats. In many estrogen-responsive tissues, TGF beta 1 has been shown to exert its effect by binding to TGF beta 1 type II receptors (T beta R II) at the cell surface. In this study, we sought to ascertain whether T beta R II is involved in TGF beta 1 action on lactotropes by determining the changes of T beta R II mRNA and protein levels and specific I-125-TGF beta 1 binding sites on the lactotropes during estrogen-induced proliferation of lactotropes in Fischer 344 rats. Double immunohistochemical procedures were employed to identify immunoreactive T beta R II in PRL-reactive cells. The majority of T beta R II-reactive cells in the anterior pituitary were observed to be lactotropes. Dual immunohistochemistry and in situ hybridization procedures also indicated that lactotropes were the major cell types containing T beta R II mRNA hybrids. Both the levels of immunoreactive T beta R II protein and in situ T beta R II mRNA hybrids in the pituitary were significantly decreased in ovariectomized rats after 15 days of estrogen treatment. Determination of I-125-TGF beta 1 binding sites in lactotropes by double immunohistochemistry and receptor autoradiography also revealed specific binding sites of I-125-TGF beta 1 in lactotropes in the anterior pituitary. I-125-TGF beta 1 binding in the anterior pituitary was also reduced following estrogen treatment in ovariectomized rats. These data suggest that down-regulation of T beta R II may be an important mechanism of estrogen action on lactotropic cell growth and PRL secretion, and further support the notion that TGF beta 1 controls lactotropic function by autocrine/paracrine mechanisms.
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WASHINGTON STATE UNIV, DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL, PULLMAN, WA 99164 USAWASHINGTON STATE UNIV, DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL, PULLMAN, WA 99164 USA
BURNS, G
SARKAR, DK
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WASHINGTON STATE UNIV, DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL, PULLMAN, WA 99164 USAWASHINGTON STATE UNIV, DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL, PULLMAN, WA 99164 USA
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WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164
GROVE, KL
COOK, VI
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WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164
COOK, VI
SPETH, RC
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WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164
机构:
WASHINGTON STATE UNIV, DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL, PULLMAN, WA 99164 USAWASHINGTON STATE UNIV, DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL, PULLMAN, WA 99164 USA
BURNS, G
SARKAR, DK
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WASHINGTON STATE UNIV, DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL, PULLMAN, WA 99164 USAWASHINGTON STATE UNIV, DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL, PULLMAN, WA 99164 USA
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WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164
GROVE, KL
COOK, VI
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WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164
COOK, VI
SPETH, RC
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WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164