Pituitary lactotrope expresses transforming growth factor beta (TGF beta) type II receptor mRNA and protein and contains I-125-TGF beta 1 binding sites

被引:48
作者
De, A
Morgan, TE
Speth, RC
Boyadjieva, N
Sarkar, DK
机构
[1] WASHINGTON STATE UNIV,DEPT VET & COMPARAT ANAT PHARMACOL & PHYSIOL,PULLMAN,WA 99164
[2] UNIV SO CALIF,DEPT BIOL SCI,ETHEL PERCY ANDRUS GERONTOL CTR,LOS ANGELES,CA 90089
关键词
D O I
10.1677/joe.0.1490019
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Transforming growth factor beta 1 (TGF beta 1) has recently been shown to be produced in the prolactin (PRL)-secreting lactotropes of the pituitary gland. TGF beta 1 inhibits lactotropic secretion and proliferation, and the production of TGF beta 1 in lactotropes is reduced during lactotropic growth following estrogen treatment in ovariectomized rats. In many estrogen-responsive tissues, TGF beta 1 has been shown to exert its effect by binding to TGF beta 1 type II receptors (T beta R II) at the cell surface. In this study, we sought to ascertain whether T beta R II is involved in TGF beta 1 action on lactotropes by determining the changes of T beta R II mRNA and protein levels and specific I-125-TGF beta 1 binding sites on the lactotropes during estrogen-induced proliferation of lactotropes in Fischer 344 rats. Double immunohistochemical procedures were employed to identify immunoreactive T beta R II in PRL-reactive cells. The majority of T beta R II-reactive cells in the anterior pituitary were observed to be lactotropes. Dual immunohistochemistry and in situ hybridization procedures also indicated that lactotropes were the major cell types containing T beta R II mRNA hybrids. Both the levels of immunoreactive T beta R II protein and in situ T beta R II mRNA hybrids in the pituitary were significantly decreased in ovariectomized rats after 15 days of estrogen treatment. Determination of I-125-TGF beta 1 binding sites in lactotropes by double immunohistochemistry and receptor autoradiography also revealed specific binding sites of I-125-TGF beta 1 in lactotropes in the anterior pituitary. I-125-TGF beta 1 binding in the anterior pituitary was also reduced following estrogen treatment in ovariectomized rats. These data suggest that down-regulation of T beta R II may be an important mechanism of estrogen action on lactotropic cell growth and PRL secretion, and further support the notion that TGF beta 1 controls lactotropic function by autocrine/paracrine mechanisms.
引用
收藏
页码:19 / 27
页数:9
相关论文
共 28 条
  • [1] TRANSFORMING GROWTH FACTOR-BETA(1)-LIKE IMMUNOREACTIVITY IN THE PITUITARY-GLAND OF THE RAT - EFFECT OF ESTROGEN
    BURNS, G
    SARKAR, DK
    [J]. ENDOCRINOLOGY, 1993, 133 (03) : 1444 - 1449
  • [2] TYPE-I RECEPTORS SPECIFY GROWTH-INHIBITORY AND TRANSCRIPTIONAL RESPONSES TO TRANSFORMING GROWTH-FACTOR-BETA AND ACTIVIN
    CARCAMO, J
    WEIS, FMB
    VENTURA, F
    WIESER, R
    WRANA, JL
    ATTISANO, L
    MASSAGUE, J
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (06) : 3810 - 3821
  • [3] CHEIFETZ S, 1988, J BIOL CHEM, V263, P17225
  • [4] INACTIVATION OF THE TYPE-II RECEPTOR REVEALS 2 RECEPTOR PATHWAYS FOR THE DIVERSE TGF-BETA ACTIVITIES
    CHEN, RH
    EBNER, R
    DERYNCK, R
    [J]. SCIENCE, 1993, 260 (5112) : 1335 - 1338
  • [5] CHIGINI N, 1994, OBSTET GYNECOLOGY, V83, P455
  • [6] IMMUNODETECTION AND QUANTITATION OF THE 2 FORMS OF TRANSFORMING GROWTH FACTOR-BETA (TGF-BETA-1 AND TGF-BETA-2) SECRETED BY CELLS IN CULTURE
    DANIELPOUR, D
    DART, LL
    FLANDERS, KC
    ROBERTS, AB
    SPORN, MB
    [J]. JOURNAL OF CELLULAR PHYSIOLOGY, 1989, 138 (01) : 79 - 86
  • [7] CLONING OF A TYPE-I TGF-BETA RECEPTOR AND ITS EFFECT OF TGF-BETA BINDING TO THE TYPE-II RECEPTOR
    EBNER, R
    CHEN, RH
    SHUM, L
    LAWLER, S
    ZIONCHECK, TF
    LEE, A
    LOPEZ, AR
    DERYNCK, R
    [J]. SCIENCE, 1993, 260 (5112) : 1344 - 1348
  • [8] GOTTSCHALL PE, 1986, P SOC EXP BIOL MED, V181, P78
  • [9] ANGIOTENSIN-II RECEPTOR DEVELOPMENT IN THE BED NUCLEUS OF THE STRIA TERMINALIS AND OTHER PERIHYPOTHALAMIC BRAIN-REGIONS OF THE FEMALE AND MALE-RAT
    GROVE, KL
    COOK, VI
    SPETH, RC
    [J]. NEUROENDOCRINOLOGY, 1992, 56 (02) : 169 - 177
  • [10] HOCK JM, 1988, CALCIFIED TISSUE INT, V43, P876