Biological and genetic differences between lung- and brain-derived isolates of maedi-visna virus

被引:29
作者
Andrésdóttir, V [1 ]
Tang, XS [1 ]
Agnarsdóttir, G [1 ]
Andrésson, OS [1 ]
Georgsson, G [1 ]
Skraban, R [1 ]
Torsteinsdóttir, S [1 ]
Rafnar, B [1 ]
Benediktsdóttir, E [1 ]
Matthíasdottir, S [1 ]
Arnadóttir, S [1 ]
Högnadóttir, S [1 ]
Pálsson, PA [1 ]
Pétursson, G [1 ]
机构
[1] Univ Iceland, Inst Expt Pathol, IS-112 Reykjavik, Iceland
关键词
lentivirus; maedi-visna virus; neurovirulence; cell tropism;
D O I
10.1023/A:1008030706308
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
During the epidemic caused by maedi-visna virus (MVV) of sheep in Iceland, the pulmonary affection, maedi, was the predominant clinical manifestation. In some hocks, however, a central nervous system (CNS) affection, visna, was the main cause of morbidity and mortality. As there is only one breed of sheep in the country, host factors did apparently not play an important role in the different clinical manifestations. To obtain some information on possible viral genetic determinants of neurotropism and neurovirulence we studied both phenotypic and genotypic properties of two maedi-visna virus strains; a strain that was originally isolated from the brain of sheep with encephalitis (visna), and another strain isolated from the lungs of a sheep suffering from pneumonia (maedi). The brain isolate was found to grow faster in sheep choroid plexus cells than the lung isolate, whereas the growth rate in macrophages was similar for the maedi and visna virus strains. Intracerebral inoculation indicated that the visna virus isolate induced more severe brain lesions than the maedi isolate. In addition, a pathogenic molecular clone derived from a visna strain (KV1772kv72/67) was tested for growth in sheep choroid plexus cells and macrophages. The molecularly cloned virus retained the fast growth rate in choroid plexus cells. The nucleotide sequence of the env gene and the U3 of the LTR was determined for the maedi strain and compared to that of the visna strains. There was an 11.7% difference in deduced amino acid sequence in the Env protein and a 6% difference in the LTR. The molecular clone KV1772kv72/67 will be a useful reagent for characterization of viral determinants of cell tropism in vitro and possibly neurovirulence in vivo.
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页码:281 / 293
页数:13
相关论文
共 41 条
[1]   ANALYSIS OF ENVELOPE CHANGES ACQUIRED BY SIV(MAC)239 DURING NEUROADAPTATION IN RHESUS MACAQUES [J].
ANDERSON, MG ;
HAUER, D ;
SHARMA, DP ;
JOAG, SV ;
NARAYAN, O ;
ZINK, MC ;
CLEMENTS, JE .
VIROLOGY, 1993, 195 (02) :616-626
[2]   NUCLEOTIDE-SEQUENCE AND BIOLOGICAL PROPERTIES OF A PATHOGENIC PROVIRAL MOLECULAR CLONE OF NEUROVIRULENT VISNA VIRUS [J].
ANDRESSON, OS ;
ELSER, JE ;
TOBIN, GJ ;
GREENWOOD, JD ;
GONDA, MA ;
GEORGSSON, G ;
ANDRESDOTTIR, V ;
BENEDIKTSDOTTIR, E ;
CARLSDOTTIR, HM ;
MANTYLA, EO ;
RAFNAR, B ;
PALSSON, PA ;
CASEY, JW ;
PETURSSON, G .
VIROLOGY, 1993, 193 (01) :89-105
[3]   THE VISNA VIRUS GENOME - EVIDENCE FOR A HYPERVARIABLE SITE IN THE ENV-GENE AND SEQUENCE HOMOLOGY AMONG LENTIVIRUS ENVELOPE PROTEINS [J].
BRAUN, MJ ;
CLEMENTS, JE ;
GONDA, MA .
JOURNAL OF VIROLOGY, 1987, 61 (12) :4046-4054
[4]   INTERACTIONS OF THE TRANSCRIPTION FACTOR AP-1 WITH THE LONG TERMINAL REPEAT OF DIFFERENT HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 STRAINS IN JURKAT, GLIAL, AND NEURONAL CELLS [J].
CANONNEHERGAUX, F ;
AUNIS, D ;
SCHAEFFER, E .
JOURNAL OF VIROLOGY, 1995, 69 (11) :6634-6642
[5]   ISOLATES OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 FROM THE BRAIN MAY CONSTITUTE A SPECIAL GROUP OF THE AIDS VIRUS [J].
CHENGMAYER, C ;
WEISS, C ;
SETO, D ;
LEVY, JA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (21) :8575-8579
[6]  
CORBOY JR, 1992, SCIENCE, V258, P1804, DOI 10.1126/science.1465618
[7]   Identification of a major co-receptor for primary isolates of HIV-1 [J].
Deng, HK ;
Liu, R ;
Ellmeier, W ;
Choe, S ;
Unutmaz, D ;
Burkhart, M ;
DiMarzio, P ;
Marmon, S ;
Sutton, RE ;
Hill, CM ;
Davis, CB ;
Peiper, SC ;
Schall, TJ ;
Littman, DR ;
Landau, NR .
NATURE, 1996, 381 (6584) :661-666
[8]   HIV-INFECTION OF HUMAN CHOROID-PLEXUS - A POSSIBLE MECHANISM OF VIRAL ENTRY INTO THE CNS [J].
FALANGOLA, MF ;
HANLY, A ;
GALVAOCASTRO, B ;
PETITO, CK .
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 1995, 54 (04) :497-503
[9]   HIV-1 Entry Cofactor: Functional cDNA Cloing of a Seven-Transmembrane, G protein-Coupled Receptor [J].
Feng, Yu ;
Broder, Christopher C. ;
Kennedy, Paul E. ;
Berger, Edward A. .
JOURNAL OF IMMUNOLOGY, 2011, 186 (11) :872-877
[10]   TROPISM OF SHEEP LENTIVIRUSES FOR MONOCYTES - SUSCEPTIBILITY TO INFECTION AND VIRUS GENE-EXPRESSION INCREASE DURING MATURATION OF MONOCYTES TO MACROPHAGES [J].
GENDELMAN, HE ;
NARAYAN, O ;
KENNEDYSTOSKOPF, S ;
KENNEDY, PGE ;
GHOTBI, Z ;
CLEMENTS, JE ;
STANLEY, J ;
PEZESHKPOUR, G .
JOURNAL OF VIROLOGY, 1986, 58 (01) :67-74