Stem cell factor and interleukin-3 induce stepwise generation of erythroid precursor cells from a basic fibroblast growth factor-dependent hematopoietic stem cell line, A-6

被引:5
作者
Anzai, H
Ikawa, Y
Atsumi, T
机构
[1] RIKEN, Tsukuba Inst, Lab Mol Cell Sci, Tsukuba, Ibaraki 3050074, Japan
[2] RIKEN, Ikawa Lab, Wako, Saitama 3510198, Japan
基金
日本科学技术振兴机构;
关键词
erythroid differentiation; GFU-e; PU.1; beta-major globin; GATA-1; F4/80; interleukin-3; stem cell factor; hematopoietic stem cell; basic fibroblast growth factor;
D O I
10.1006/bbrc.2001.4674
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A multipotent; immature myeloid cell population was produced from a basic fibroblast growth factor (bFGF)-dependent hematopoietic stem cell line, A-6, when cultured with stem cell factor (SCF) replacing bFGF. Those cells were positive for stem cell markers, c-kit and CD34, and a myeloid cell marker, F4/80. Some cell fractions were also positive for Mac-1, a macrophage marker cap Gr-1, a granulocytic maker, but negative for an erythroid marker TER119. They also showed the expression of mRNA for the myeloid-specific PU.1 but did not that for the erythroid-specific GATA-1. Among various cytokines, interleukin-3 (IL-3) induced erythroid precursor cells that expressed the erythroid-specific GATA-1 and beta -major globin. The quantitative analysis showed that erythroid precursor cells mere newly produced from the immature myeloid cells by cultivation with IL-3. SCF and IL-3 induced stepwise generation of erythroid precursor cells from an A-6 hennatopoietic stem cell line. (C) 2001 Academic Press.
引用
收藏
页码:940 / 946
页数:7
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