p38MAP Kinase activity is required for human primary adipocyte differentiation

被引：80

作者：

Aouadi, M. ^{[1
,2
]}

Jager, J. ^{[1
,2
]}

Laurent, K. ^{[1
,2
]}

Gonzalez, T. ^{[1
,2
]}

Cormont, M. ^{[1
,2
]}

Binetruy, B. ^{[3
,4
]}

Le Marchand-Brustel, Y. ^{[1
,2
,5
]}

Tanti, J. -F. ^{[1
,2
]}

Bost, F. ^{[1
,2
]}

机构：

[1] INSERM, Fac Med, U568, IFR50, F-06107 Nice 02, France

[2] Univ Nice Sophia Antipolis, Fac Med, F-06107 Nice, France

[3] INSERM, U626, F-13385 Marseille, France

[4] Univ Aix Marseille 2, Fac Med, F-13385 Marseille, France

[5] CHU Nice, F-06102 Nice, France

来源：

FEBS LETTERS | 2007年 / 581卷 / 29期

基金：

澳大利亚研究理事会;

关键词：

adipogenesis; p38MAPK; c/EBPbeta; PPAR gamma; human preadipocytes; visceral adipose tissue; subcutaneous adipose tissue;

D O I：

10.1016/j.febslet.2007.10.064

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Little is known about the role of p38MAPK in human adipocyte di. fferentiation. Here we showed that p38MAPK activity increases during human preadipocytes differentiation. Pharmacological inhibition of p38MAPK during adipocyte differentiation of primary human preadipocytes markedly reduced triglycerides accumulation and adipocyte markers expression. Cell cycle arrest or proliferation was not a. ffected by p38MAPK inhibition. Although induction of C/EBP beta was not altered by the p38MAPK inhibitor, its phosphorylation on Threonine 188 was decreased as well as PPAR gamma expression. These results indicate that p38MAPK plays a positive role in human adipogenesis through regulation of C/EBP beta and PPAR gamma factors. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：5591 / 5596

页数：6

共 19 条

[1] Role of MAPKs in development and differentiation: lessons from knockout mice [J].

Aouadi, M. ;

Binetruy, B. ;

Caron, L. ;

Le Marchand-Brustel, Y. ;

Bost, F. .

BIOCHIMIE, 2006, 88 (09) :1091-1098

[2] Inhibition of p38MAPK increases adipogenesis from embryonic to adult stages [J].

Aouadi, M ;

Laurent, K ;

Prot, M ;

Le Marchand-Brustel, Y ;

Binétruy, B ;

Bost, F .

DIABETES, 2006, 55 (02) :281-289

[3] INHIBITION OF ADIPOGENESIS BY THE STRESS-INDUCED PROTEIN CHOP (GADD153) [J].

BATCHVAROVA, N ;

WANG, XZ ;

RON, D .

EMBO JOURNAL, 1995, 14 (19) :4654-4661

[4] The extracellular signal-regulated kinase isoform ERK1 is specifically required for in vitro and in vivo adipogenesis [J].

Bost, F ;

Aouadi, M ;

Caron, L ;

Even, P ;

Belmonte, N ;

Prot, M ;

Dani, C ;

Hofman, P ;

Pagès, G ;

Pouysségur, J ;

Le Marchand-Brustel, Y ;

Binétruy, B .

DIABETES, 2005, 54 (02) :402-411

[5] The role of MAPKs in adipocyte differentiation and obesity [J].

Bost, F ;

Aouadi, M ;

Caron, L ;

Binétruy, B .

BIOCHIMIE, 2005, 87 (01) :51-56

[6] Retinoic acid activation of the ERK pathway is required for embryonic stem cell commitment into the adipocyte lineage [J].

Bost, F ;

Caron, L ;

Marchetti, I ;

Dani, C ;

Le Marchand-Brustel, Y ;

Binétruy, B .

BIOCHEMICAL JOURNAL, 2002, 361 :621-627

[7] Constitutively active mitogen-activated protein kinase kinase 6 (MIKK6) or salicylate induces spontaneous 3T3-L1 adipogenesis [J].

Engelman, JA ;

Berg, AH ;

Lewis, RY ;

Lin, AN ;

Lisanti, MP ;

Scherer, PE .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (50) :35630-35638

[8] Specific inhibitors of p38 mitogen-activated protein kinase block 3T3-L1 adipogenesis [J].

Engelman, JA ;

Lisanti, MP ;

Scherer, PE .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (48) :32111-32120

[9] Gene expression profile of omental adipose tissue in human obesity [J].

Gómez-Ambrosi, J ;

Catalán, V ;

Diez-Caballero, A ;

Martínez-Cruz, LA ;

Gil, MJ ;

García-Foncillas, J ;

Cienfuegoas, JA ;

Salvador, J ;

Mato, JM ;

Fruhbeck, G .

FASEB JOURNAL, 2004, 18 (01) :215-+

[10] A potential role for the nuclear factor of activated T cells family of transcriptional regulatory proteins in adipogenesis [J].

Ho, IC ;

Kim, JHJ ;

Rooney, JW ;

Spiegelman, BM ;

Glimcher, LH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (26) :15537-15541

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