Identification by high-performance liquid chromatography diode array detection mass spectrometry and quantification by high-performance liquid chromatography UV absorbance detection of active constituents of Hypericum perforatum

被引：206

作者：

Brolis, M ^{[1
]}

Gabetta, B ^{[1
]}

Fuzzati, N ^{[1
]}

Pace, R ^{[1
]}

Panzeri, F ^{[1
]}

Peterlongo, F ^{[1
]}

机构：

[1] Indena SPA, Lab Ricerca & Sviluppo, I-20090 Milan, Italy

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 825卷 / 01期

关键词：

Hypericum perforatum; flavonoids; naphthodianthrones; phloroglucinols; rutin; chlorogenic acid;

D O I：

10.1016/S0021-9673(98)00697-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Hypericum perforatum is a medicinal plant which has been known in traditional medicine as an anti-inflammatory and healing agent. Nowadays, the alcoholic extract of its aerial parts finds wide application for its antidepressant activity. A high-performance liquid chromatography (HPLC) method for the identification of its constituents using a wide pore RP-18 column and a water-methanol-acetonitrile-phosphoric acid mobile phase system was developed. The identification of its flavonoid, naphthodianthrone and phloroglucinol constituents was performed using combined HPLC-diode array detection (DAD) analysis, HPLC-thermospray and HPLC-electrospray mass spectrometry. Chlorogenic acid, quercetin, quercitrin, isoquercitrin, rutin, hyperoside, I3,II8-biapigenin, pseudohypericin, hypericin, hyperforin and adhyperforin were separated by an aqueous phosphoric acid-acetonitrile-methanol gradient within 50 min. The quantification of the above constituents was performed using rutin as an external standard. (C) 1998 Elsevier Science BN. All rights reserved.

引用

页码：9 / 16

页数：8

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