The JIL-1 kinase regulates the structure of Drosophila polytene chromosomes

被引:54
作者
Deng, H [1 ]
Zhang, WG [1 ]
Bao, XM [1 ]
Martin, JN [1 ]
Girton, J [1 ]
Johansen, J [1 ]
Johansen, KM [1 ]
机构
[1] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA
关键词
D O I
10.1007/s00412-005-0006-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The JIL-1 kinase localizes to interband regions of Drosophila polytene chromosomes and phosphorylates histone H3 Ser10. Analysis of JIL-1 hypomorphic alleles demonstrated that reduced levels of JIL-1 protein lead to global changes in polytene chromatin structure. Here we have performed a detailed ultrastructural and cytological analysis of the defects in JIL-1 mutant chromosomes. We show that all autosomes and the female X chromosome are similarly affected, whereas the defects in the male X chromosome are qualitatively different. In polytene autosomes, loss of JIL-1 leads to misalignment of interband chromatin fibrils and to increased ectopic contacts between nonhomologous regions. Furthermore, there is an abnormal coiling of the chromosomes with an intermixing of euchromatic regions and the compacted chromatin characteristic of banded regions. In contrast, coiling of the male X polytene chromosome was not observed. Instead, the shortening of the male X chromosome appeared to be caused by increased dispersal of the chromatin into a diffuse network without any discernable banded regions. To account for the observed phenotypes we propose a model in which JIL-1 functions to establish or maintain the parallel alignment of interband chromosome fibrils as well as to repress the formation of contacts and intermingling of nonhomologous chromatid regions.
引用
收藏
页码:173 / 182
页数:10
相关论文
共 28 条
[1]   ELEMENTARY STRUCTURES IN POLYTENE CHROMOSOMES OF DROSOPHILA-MELANOGASTER [J].
ANANIEV, EV ;
BARSKY, VE .
CHROMOSOMA, 1985, 93 (02) :104-112
[2]   ECTOPIC PAIRING OF CHROMOSOME REGIONS CONTAINING CHEMICALLY SIMILAR DNA [J].
BARR, HJ ;
ELLISON, JR .
CHROMOSOMA, 1972, 39 (01) :53-+
[3]   Visualizing chromosome dynamics with GFP [J].
Belmont, AS .
TRENDS IN CELL BIOLOGY, 2001, 11 (06) :250-257
[4]   ACETYLATED HISTONE H4 ON THE MALE X-CHROMOSOME IS ASSOCIATED WITH DOSAGE COMPENSATION IN DROSOPHILA [J].
BONE, JR ;
LAVENDER, J ;
RICHMAN, R ;
PALMER, MJ ;
TURNER, BM ;
KURODA, MI .
GENES & DEVELOPMENT, 1994, 8 (01) :96-117
[5]   A monomeric red fluorescent protein [J].
Campbell, RE ;
Tour, O ;
Palmer, AE ;
Steinbach, PA ;
Baird, GS ;
Zacharias, DA ;
Tsien, RY .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (12) :7877-7882
[6]   A His2AvDGFP fusion gene complements a lethal His2AvD mutant allele and provides an in vivo marker for Drosophila chromosome behavior [J].
Clarkson, M ;
Saint, R .
DNA AND CELL BIOLOGY, 1999, 18 (06) :457-462
[7]   Mechanisms of HP1-mediated gene silencing in Drosophila [J].
Danzer, JR ;
Wallrath, LL .
DEVELOPMENT, 2004, 131 (15) :3571-3580
[8]   The ISWI chromatin-remodeling protein is required for gene expression and the maintenance of higher order chromatin structure in vivo [J].
Deuring, R ;
Fanti, L ;
Armstrong, JA ;
Sarte, M ;
Papoulas, O ;
Prestel, M ;
Daubresse, G ;
Verardo, M ;
Moseley, SL ;
Berloco, M ;
Tsukiyama, T ;
Wu, C ;
Pimpinelli, S ;
Tamkun, JW .
MOLECULAR CELL, 2000, 5 (02) :355-365
[9]   Identification of the Drosophila interband-specific protein Z4 as a DNA-binding zinc-finger protein determining chromosomal structure [J].
Eggert, H ;
Gortchakov, A ;
Saumweber, H .
JOURNAL OF CELL SCIENCE, 2004, 117 (18) :4253-4264
[10]  
GHOSH M, 1987, DROS INF SERV, V66, P63