Stepwise synthesis of cellodextrins assisted by a mutant cellulase

被引：22

作者：

Fort, S

Christiansen, L

Schülein, M

Cottaz, S

Driguez, H

机构：

[1] CNRS, Ctr Rech Macromol Vegetales, F-38041 Grenoble 9, France

[2] Novo Nordisk AS, DK-2880 Bagsvaerd, Denmark

来源：

ISRAEL JOURNAL OF CHEMISTRY | 2000年 / 40卷 / 3-4期

关键词：

D O I：

10.1560/5NCY-L9K2-JND4-MB6Q

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A 4(II)-protected alpha -cellobiosyl fluoride is an efficient donor for the enzymatic stepwise condensations onto methyl P-D-glycoside accepters. These reactions, catalyzed by the Cel7B E197A glycosynthase from Humicola insolens, gave cellodextrins from degree of polymerization 3 to 6 in high yields.

引用

收藏

页码：217 / 221

页数：5

相关论文

共 26 条

[1] A bifunctionalized fluorogenic tetrasaccharide as a substrate to study cellulases [J].

Armand, S ;

Drouillard, S ;

Schulein, M ;

Henrissat, B ;

Driguez, H .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (05) :2709-2713

[2]

ERICKSSON KEL, 1998, ACS S SERIES, V687

[3] SELECTIVE CLEAVAGE OF ANOMERIC ACETYL GROUPS OF ACETYLATED GLYCOSYL RESIDUES BY HYDRAZINE [J].

EXCOFFIER, G ;

GAGNAIRE, D ;

UTILLE, JP .

CARBOHYDRATE RESEARCH, 1975, 39 (02) :368-373

[4] Isolation, modification, and NMR assignments of a series of cellulose oligomers [J].

Flugge, LA ;

Blank, JT ;

Petillo, PA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (31) :7228-7238

[5] Highly efficient synthesis of β(1→4)-oligo- and -polysaccharides using a mutant cellulase [J].

Fort, S ;

Boyer, V ;

Greffe, L ;

Davies, G ;

Moroz, O ;

Christiansen, L ;

Schülein, M ;

Cottaz, S ;

Driguez, H .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (23) :5429-5437

[6] SIMPLE SYNTHESIS OF GLYCOSYL FLUORIDES [J].

HAYASHI, M ;

HASHIMOTO, S ;

NOYORI, R .

CHEMISTRY LETTERS, 1984, (10) :1747-1750

[7]

HRMOVA M, 2000, 29 INT CARB S HAMB A

[8] SYNTHETIC STUDIES ON MUCOPOLYSACCHARIDES .2. DISCRIMINATION BETWEEN THE 2,3-HYDROXYL AND THE 2',3'-HYDROXYL GROUPS OF MALTOSE AND CELLOBIOSE THROUGH THEIR SPECIFIC PROTECTION [J].

ICHIKAWA, Y ;

MANAKA, A ;

KUZUHARA, H .

CARBOHYDRATE RESEARCH, 1985, 138 (01) :55-64

[9] NOVEL METHOD FOR POLYSACCHARIDE SYNTHESIS USING AN ENZYME - THE 1ST INVITRO SYNTHESIS OF CELLULOSE VIA A NONBIOSYNTHETIC PATH UTILIZING CELLULASE AS CATALYST [J].

KOBAYASHI, S ;

KASHIWA, K ;

KAWASAKI, T ;

SHODA, S .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (08) :3079-3084

[10] Glycosynthases: Mutant glycosidases for oligosaccharide synthesis [J].

Mackenzie, LF ;

Wang, QP ;

Warren, RAJ ;

Withers, SG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (22) :5583-5584