Engineering tomato for resistance to tomato leaf curl disease using viral rep gene sequences

被引：15

作者：

Praveen, S ^{[1
]}

Kushwaha, CM ^{[1
]}

Mishra, AK ^{[1
]}

Singh, V ^{[1
]}

Jain, RK ^{[1
]}

Varma, A ^{[1
]}

机构：

[1] Indian Agr Res Inst, Adv Ctr Plant Virol, Div Plant Pathol, New Delhi 110012, India

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2005年 / 83卷 / 03期

关键词：

RNA-mediated virus resistance; tomato; Tomato leaf curl virus; transformation;

D O I：

10.1007/s11240-005-7858-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Transgenic tomato resistant to tomato leaf curl disease (ToLCD) using replicase (rep) gene sequences of Tomato leaf curl virus in antisense orientation were developed via Agrobacterium-mediated transformation. A binary vector carrying the antisense rep gene (untranslatable full length sequence, 1086 bp) along with the npt II gene was used for transformation. High level of resistance and inheritability of the transgene was observed up to T-2 stage following challenge inoculation with the virus. The mechanism of resistance appears RNA-mediated, since the plants carried the untranslatable antisense rep gene. Progeny analysis of these plants showed classical Mendelian pattern of inheritance in two of the six transgenic lines having single transgene insertion.

引用

页码：311 / 318

页数：8

共 36 条

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