T-cell receptor gene usage in patients with fibrosing alveolitis and control subjects

被引:8
作者
Lympany, PA
Southcott, AM
Welsh, KI
Black, CM
Boylston, AW
du Bois, RM
机构
[1] Univ London Imperial Coll Sci Technol & Med, Dept Occupat & Environm Med, Interstitial Lung Dis Unit, London SW3 6LR, England
[2] Churchill Hosp, Transplantat Ctr, Oxford OX3 7LJ, England
[3] Royal Free Hosp, Royal Free Acad Unit Rheum & Connec Tiss Dis, London NW3 2PF, England
[4] St James Univ Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England
关键词
cryptogenic fibrosing alveolitis; fibrosing alveolitis; fibrosing alveolitis systemic sclerosis; T-cell receptor;
D O I
10.1046/j.1365-2362.1999.00434.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background Fibrosing alveolitis is characterized by inflammation, fibrosis and increased numbers of activated CD4(+) T-cells in the lower respiratory tract. The aims of this study were to compare the T-cell antigen receptor repertoire in the lungs of subjects with fibrosing alveolitis systemic sclerosis (FASSc) with cryptogenic fibrosing alveolitis (CFA) and normal control subjects, to determine whether FASSc is driven by a specific T-cell trigger and is determined by a T-cell driven immune response, and to assess the clonality of CD4+ and CD8(+) TcR usage in subjects with FASSc. Materials and methods We used reverse transcription polymerase chain reaction with specific V alpha- and V beta-chain primers to identify the TcR gene usage in biopsy material, bronchoalveolar lavage fluid or peripheral blood from our subjects. Results We found individual-specific restriction of V alpha- and V beta-chain usage in lung biopsies from patients and control subjects. To establish whether this was due to expression bias in the CD4(+) or CD8(+) T-cells and was restricted to the lung, the alpha beta-T-cell receptor chain usage was assessed in T-cell subsets separated from the lungs of patients with fibrosing alveolitis and was compared with that of the peripheral blood. There was no consistent difference in the expression of any variable family chain among the population studied, although there was a significant difference between lung and peripheral blood lymphocyte V beta-families in CD8(+) T-cells (P = 0.0007). Conclusion We conclude that there is individual TcR V alpha- and V beta-expression bias in subjects with fibrosing alveolitis.
引用
收藏
页码:173 / 181
页数:9
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