Analysis of the thromboxane/prostacyclin balance in human urine by gas chromatography selected ion monitoring: Abnormalities in diabetics

被引:22
作者
Hishinuma, T
Yu, GSP
Takabatake, M
Nakagawa, Y
Ito, K
Nishikawa, M
Ishibashi, M
Suzuki, K
Matsumoto, M
Toyoda, T
Mizugaki, M
机构
[1] TOHOKU UNIV HOSP,DEPT PHARMACEUT SCI,AOBA KU,SENDAI,MIYAGI 98077,JAPAN
[2] TAKATA SEIYAKU CO LTD,RES LABS,OMIYA,JAPAN
[3] TOHOKU KOSEINENKIN HOSP,DEPT INTERNAL MED,MIYAGINO KU,SENDAI,MIYAGI 983,JAPAN
[4] TOHOKU UNIV,DEPT INTERNAL MED 3,AOBA KU,SENDAI,MIYAGI 98077,JAPAN
来源
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1996年 / 54卷 / 06期
关键词
D O I
10.1016/S0952-3278(96)90029-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We microanalyzed 2,3-dinor-6-keto-prostaglandin F-1 alpha (2,3-dinor-6-keto-PGF(1 alpha) 1)and 11-dehydro-thromboxane B-2 (11-dehydro-TXB(2), 2) in human urine. Samples containing a [H-2(4)]-analogue as an internal standard were extracted by chromatography using Sep Pak tC18 and silica gel. The compounds were then analysed by means of the lactone ring opening reaction and dimethylisopropylsilylation. The conversion of 1 to 1-methyl ester (ME)-propylamide (PA)-9,12,15-dimethylisopropylsilyl (DMIPS) ether derivative and of 2 to 1-ME-6-methoxime (MO)-9,12,15-tris-DMIPS ether derivative was followed by gas chromatography/selected ion monitoring (GC/SIM). Interfering substances from the urine matrix were eliminated during GC/SIM analysis using a DB-5 column. We were able to detect 1 (222-1031 pg/mg creatinine) and 2 (18-155 pg/mg creatinine) in human urine. Furthermore, the thromboxane/prostacyclin (IX/PGI) ratio in the urine of diabetics was higher than that of healthy volunteers. This method can be used to determine the TX/PGI balance in human urine.
引用
收藏
页码:445 / 449
页数:5
相关论文
共 21 条
[1]  
DAVIS TME, 1979, LANCET, V2, P789
[2]   INVITRO VENOUS PROSTACYCLIN PRODUCTION, PLASMA 6-KETO-PROSTAGLANDIN-F1-ALPHA CONCENTRATIONS, AND DIABETIC-RETINOPATHY [J].
DAVIS, TME ;
BOWN, E ;
FINCH, DR ;
MITCHELL, MD ;
TURNER, RC .
BMJ-BRITISH MEDICAL JOURNAL, 1981, 282 (6272) :1259-1262
[3]  
DOLLERY CT, 1979, LANCET, V2, P1365
[4]   EICOSAPENTANOIC ACID AND PREVENTION OF THROMBOSIS AND ATHEROSCLEROSIS [J].
DYERBERG, J ;
BANG, HO ;
STOFFERSEN, E ;
MONCADA, S ;
VANE, JR .
LANCET, 1978, 2 (8081) :117-119
[5]   FORMATION OF PROSTACYCLIN AND THROMBOXANE IN MAN AS MEASURED BY THE MAIN URINARY METABOLITES [J].
FISCHER, S ;
BERNUTZ, C ;
MEIER, H ;
WEBER, PC .
BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 876 (02) :194-199
[6]  
FitzGerald G A, 1985, Adv Prostaglandin Thromboxane Leukot Res, V15, P87
[7]   INCREASED PROSTACYCLIN BIOSYNTHESIS IN PATIENTS WITH SEVERE ATHEROSCLEROSIS AND PLATELET ACTIVATION [J].
FITZGERALD, GA ;
SMITH, B ;
PEDERSEN, AK ;
BRASH, AR .
NEW ENGLAND JOURNAL OF MEDICINE, 1984, 310 (17) :1065-1068
[8]   EFFECT OF INSULIN-TREATMENT ON PROSTACYCLIN IN EXPERIMENTAL DIABETES [J].
HARRISON, HE ;
REECE, AH ;
JOHNSON, M .
DIABETOLOGIA, 1980, 18 (01) :65-68
[9]  
HARRISON HE, 1979, LANCET, V1, P325
[10]   ANALYSIS OF 11-DEHYDROTHROMBOXANE B-2 IN HUMAN URINE IMPROVED BY USE OF GAS-CHROMATOGRAPHY HIGH-RESOLUTION SELECTED-ION MONITORING WITH O-18(2)-LABELED ANALOG AS AN INTERNAL STANDARD [J].
ISHIBASHI, M ;
NAKAGAWA, Y ;
HARIMA, N ;
HISHINUMA, T ;
MIZUGAKI, M .
BIOLOGICAL MASS SPECTROMETRY, 1994, 23 (10) :612-620