Development of a DNA sensor based on alkanethiol self-assembled monolayer-modified electrodes

被引：29

作者：

Loaiza, OA ^{[1
]}

Campuzano, S ^{[1
]}

López-Berlanga, M ^{[1
]}

Pedrero, MA ^{[1
]}

Pingarrón, JM ^{[1
]}

机构：

[1] Univ Complutense Madrid, Fac Ciencias Quim, Dept Quim Analit, E-28040 Madrid, Spain

来源：

SENSORS | 2005年 / 5卷 / 6-10期

关键词：

calf thymus DNA; self-assembled monolayers; hybridization; denaturation; methylene blue;

D O I：

10.3390/s5060344

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An electrochemical DNA biosensor based on recognition of double or single stranded DNA (ds-DNA/ss-DNA) immobilised on a self-assembled modified gold electrode is presented for denaturalisation and hybridisation detection. DNA is covalently bond on a self assembled 3-mercaptopropionic acid monolayer by using water soluble N-3( dimethylaminopropyl)-N'ethylcarbodiimide hydrochloride (EDC) and N-hydroxisulfosuccinimide ( NHSS) as linkers. The interaction between the immobilised DNA and methylene blue (MB) is investigated using square wave voltammetry (SWV). The increase or diminution of peak currents of the MB upon the hybridisation or denaturalisation event at the modified electrode surface is studied.

引用

页码：344 / 363

页数：20

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