AMID: new insights on its intracellular localization and expression at apoptosis

被引：29

作者：

Bilyy, Rostyslav ^{[1
]}

Kit, Yuriy ^{[1
]}

Hellman, Ulf ^{[2
]}

Stoika, Rostyslav ^{[1
]}

机构：

[1] Natl Acad Sci Ukraine, Inst Cell Biol, Dept Regulat Cell Proliferat & Apoptosis, UA-79005 Lvov, Ukraine

[2] Uppsala Univ, Ludwig Inst Canc Res, Uppsala, Sweden

来源：

APOPTOSIS | 2008年 / 13卷 / 05期

关键词：

AMID; AIF; apoptosis; plasma membrane; glycosylation; lectin; MALDI-TOF MS;

D O I：

10.1007/s10495-008-0198-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

AMID ((a) under bar poptosis-inducing factor (AIF)-like (m) under bar itochondrion-associated (i) under bar nducer of (d) under bar eath) is a poorly studied member of the AIF family; despite the given name AMID, predicting its association with mitochondria, its real cellular localization, as well as its role and changes during apoptosis are currently unclear. By means of MALDI-TOF mass spectrometry, we have identified as AMID (accession number AAH38129, sequence coverage 31%) the protein isolated by Pisum sativum lectin-affinity chromatography from the plasma membrane fraction of apoptotic murine leukemia L1210 cells, lacking in the intact cells. The obtained results suggest its possible glycosylation that was further suggested by finding N-glycosylation sequon in the signal peptide of AMID protein (in silica), and by predicting transmembrane localization of its N-terminal part. Using monoclonal antibodies to AMID, we demonstrated an increased expression of AMID in human leukemia Jurkat T-cells after apoptosis induction. Immunocytochemical study suggested its association to the plasma membrane.

引用

页码：729 / 732

页数：4

共 13 条

[1] In vivo expression and characteristics of novel α-D-mannose-rich glycoprotein markers of apoptotic cells [J].

Bilyy, R ;

Kit, Y ;

Hellman, U ;

Tryndyak, V ;

Kaminskyy, V ;

Stoika, R .

CELL BIOLOGY INTERNATIONAL, 2005, 29 (11) :920-928

[2] Lectinocytochemical detection of apoptotic murine leukemia L1210 cells [J].

Bilyy, RO ;

Stoika, RS .

CYTOMETRY PART A, 2003, 56A (02) :89-95

[3] Search for novel cell surface markers of apoptotic cells [J].

Bilyy, Rostyslav ;

Stoika, Rostyslav .

AUTOIMMUNITY, 2007, 40 (04) :249-253

[4] N-Terminal myristoylation predictions by ensembles of neural networks [J].

Bologna, G ;

Yvon, C ;

Duvaud, S ;

Veuthey, AL .

PROTEOMICS, 2004, 4 (06) :1626-1632

[5]

Chekhun V. F., 2003, Ukrainskii Biokhimicheskii Zhurnal, V75, P120

[6]

Chorna I, 2004, EXP ONCOL, V26, P111

[7]

Hellman U, 2000, EXS, V88, P43

[8] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[9] The p53-inducible apoptotic protein AMID is not required for normal development and tumor suppression [J].

Mei, J ;

Webb, S ;

Zhang, B ;

Shu, HB .

ONCOGENE, 2006, 25 (06) :849-856

[10] A novel p53-inducible apoptogenic gene, PRG3, encodes a homologue of the apoptosis-inducing factor (AIF) [J].

Ohiro, Y ;

Garkavtsev, I ;

Kobayashi, S ;

Sreekumar, KR ;

Nantz, R ;

Higashikubo, BT ;

Duffy, SL ;

Higashikubo, R ;

Usheva, A ;

Gius, D ;

Kley, N ;

Horikoshi, N .

FEBS LETTERS, 2002, 524 (1-3) :163-171

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