Differential pro-inflammatory effects of metal oxide nanoparticles and their soluble ions in vitro and in vivo; zinc and copper nanoparticles, but not their ions, recruit eosinophils to the lungs

被引:196
作者
Cho, Wan-Seob
Duffin, Rodger
Poland, Craig A.
Duschl, Albert [2 ]
Oostingh, Gertie Janneke [2 ]
MacNee, William
Bradley, Mark [3 ]
Megson, Ian L. [4 ]
Donaldson, Ken [1 ]
机构
[1] Univ Edinburgh, Queens Med Res Inst, Ctr Inflammat Res, Edinburgh EH16 4TJ, Midlothian, Scotland
[2] Salzburg Univ, Dept Mol Biol, A-5020 Salzburg, Austria
[3] Univ Edinburgh, Sch Chem, Edinburgh, Midlothian, Scotland
[4] Ctr Hlth Sci, UHI Dept Diabet & Cardiovasc Sci, Free Rad Res Facil, Inverness, Scotland
基金
英国医学研究理事会;
关键词
Metal oxide nanoparticles; water-soluble ions; aqueous extract; A549; cells; instillation; lung inflammation; NF-KAPPA-B; EPITHELIAL-CELLS; INTRATRACHEAL INSTILLATION; PARTICLES; TOXICITY; CYTOTOXICITY; RELEASE; FINE; RATS; IL-8;
D O I
10.3109/17435390.2011.552810
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Nickel, zinc, and copper oxide nanoparticles (NiONP, ZnONP, and CuONP) and their aqueous extracts (AEs) were applied to A549 lung epithelial cells to determine the cytotoxicity, IL-8 production, and activation of transcription factors. Nanoparticles (NPs) and their AEs were also instilled into rat lungs to evaluate acute and chronic inflammatory effects. In vitro AEs had specific effects; for example NiOAE had no effect and ZnOAE affected all parameters measured. NPs themselves all had cytotoxic effects but only ZnONP and CuONP impacted pro-inflammatory endpoints. The inflammatory cells in the BAL were also different from AEs and NPs with ZnONP and CuONP recruiting eosinophils and neutrophils whilst ZnOAE and CuOAE elicited only mild neutrophilic inflammation that had resolved by four weeks. NiONP recruited neutrophils only whilst NiOAE did not cause any inflammation. Understanding differences in the toxic role of the ionic components of metal oxide NPs will contribute to full hazard identification and characterisation.
引用
收藏
页码:22 / 35
页数:14
相关论文
共 32 条
[1]   Manufacture and use of nanomaterials: current status in the UK and global trends [J].
Aitken, R. J. ;
Chaudhry, M. Q. ;
Boxall, A. B. A. ;
Hull, M. .
OCCUPATIONAL MEDICINE-OXFORD, 2006, 56 (05) :300-306
[2]   The use of nanocrystals in biological detection [J].
Alivisatos, P .
NATURE BIOTECHNOLOGY, 2004, 22 (01) :47-52
[3]   Optimized dispersion of nanoparticles for biological in vitro and in vivo studies [J].
Bihari, Peter ;
Vippola, Minnamari ;
Schultes, Stephan ;
Praetner, Marc ;
Khandoga, Alexander G. ;
Reichel, Christoph A. ;
Coester, Conrad ;
Tuomi, Timo ;
Rehberg, Markus ;
Krombach, Fritz .
PARTICLE AND FIBRE TOXICOLOGY, 2008, 5 (1)
[4]   Interaction between nanoparticles and cytokine proteins: impact on protein and particle functionality [J].
Brown, David M. ;
Dickson, Claire ;
Duncan, Paul ;
Al-Attili, Furat ;
Stone, Vicki .
NANOTECHNOLOGY, 2010, 21 (21)
[5]   Effects of Metals within Ambient Air Particulate Matter (PM) on Human Health [J].
Chen, Lung Chi ;
Lippmann, Morton .
INHALATION TOXICOLOGY, 2009, 21 (01) :1-31
[6]   Metal Oxide Nanoparticles Induce Unique Inflammatory Footprints in the Lung: Important Implications for Nanoparticle Testing [J].
Cho, Wan-Seob ;
Duffin, Rodger ;
Poland, Craig A. ;
Howie, Sarah E. M. ;
MacNee, William ;
Bradley, Mark ;
Megson, Ian L. ;
Donaldson, Ken .
ENVIRONMENTAL HEALTH PERSPECTIVES, 2010, 118 (12) :1699-1706
[7]   CATALYSIS Individual nanoparticles in action [J].
Gates, Bruce C. .
NATURE NANOTECHNOLOGY, 2008, 3 (10) :583-584
[8]   Histone acetylation regulates epithelial IL-8 release mediated by oxidative stress from environmental particles [J].
Gilmour, PS ;
Rahman, I ;
Donaldson, K ;
MacNee, W .
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2003, 284 (03) :L533-L540
[9]   Induction of inflammation in vascular endothelial cells by metal oxide nanoparticles: Effect of particle composition [J].
Gojova, Andrea ;
Guo, Bing ;
Kota, Rama S. ;
Rutledge, John C. ;
Kennedy, Ian M. ;
Barakat, Abdul I. .
ENVIRONMENTAL HEALTH PERSPECTIVES, 2007, 115 (03) :403-409
[10]   Signal transduction in monocytes: the role of zinc ions [J].
Haase, Hajo ;
Rink, Lothar .
BIOMETALS, 2007, 20 (3-4) :579-585