Acceptable performance of VITEK 2 system to detect extended-spectrum β-lactamases in clinical isolates of Escherichia coli:: a comparative study of phenotypic commercial methods and NCCLS guidelines

被引:10
作者
Sorlózano, A [1 ]
Gutiérrez, J [1 ]
Piédrola, G [1 ]
Soto, MJ [1 ]
机构
[1] Univ Granada, San Cecilio Univ Hosp, Sch Med, Dept Microbiol, E-18012 Granada, Spain
关键词
Escherichia coli; ESBL; Etest; VITEK; 2;
D O I
10.1016/j.diagmicrobio.2004.11.001
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The disk approximation method, Etest (AB Biodisk, Solna, Sweden), and VITEK 2 system (bioMerieux, Marcy l'Etoile, France) were used to study 399 extended-spectrum beta-lactamase (ESBL)-producing (115 strains) and non-ESBL-producing (284 strains) clinical isolates of Escherichia coli after recommended procedures. Comparative study of the phenotypic findings yielded data on the sensitivity, specificity, and positive and negative predictive values and performance of each method. The sensitivity (100% using 2 substrates), specificity (99.3-100%), and predictive values of the disk approximation, Etest, and VITEK 2 methods were similar. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:191 / 193
页数:3
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