Validation of a DNA IQ™-based extraction method for TECAN robotic liquid handling workstations for processing casework

被引：25

作者：

Fregeau, Chantal J. ^{[1
]}

Lett, C. Marc ^{[2
]}

Fourney, Ron M. ^{[1
]}

机构：

[1] Royal Canadian Mounted Police, Natl Serv & Res, Ottawa, ON K1G 3M8, Canada

[2] Royal Canadian Mounted Police, Forens Lab Serv Biol, Ottawa, ON K1G 3M8, Canada

来源：

FORENSIC SCIENCE INTERNATIONAL-GENETICS | 2010年 / 4卷 / 05期

关键词：

Automation; DNA typing; Casework; Magnetic beads; DNA IQ; Fixed tips; POLYMERASE-CHAIN-REACTION; FORENSIC CASEWORK; PCR AMPLIFICATION; PURIFICATION; SAMPLES; AUTOMATION; OPTIMIZATION; BACTERIAL; SYSTEMS; KIT;

D O I：

10.1016/j.fsigen.2009.11.001

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A semi-automated DNA extraction process for casework samples based on the Promega DNA IQ (TM) system was optimized and validated on TECAN Genesis 150/8 and Freedom EVO robotic liquid handling stations configured with fixed tips and a TECAN TE-Shake (TM) unit. The use of an orbital shaker during the extraction process promoted efficiency with respect to DNA capture, magnetic bead/DNA complex washes and DNA elution. Validation studies determined the reliability and limitations of this shaker-based process. Reproducibility with regards to DNA yields for the tested robotic workstations proved to be excellent and not significantly different than that offered by the manual phenol/chloroform extraction. DNA extraction of animal: human blood mixtures contaminated with soil demonstrated that a human profile was detectable even in the presence of abundant animal blood. For exhibits containing small amounts of biological material, concordance studies confirmed that DNA yields for this shaker-based extraction process are equivalent or greater to those observed with phenol/chloroform extraction as well as our original validated automated magnetic bead percolation-based extraction process. Our data further supports the increasing use of robotics for the processing of casework samples. Crown Copyright (C) 2009 Published by Elsevier Ireland Ltd. All rights reserved.

引用

页码：292 / 304

页数：13

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