The synthesis of 7-substituted-3-dinitrostyryl cephalosporins and their ability for detecting extended spectrum β-lactamases (ESBLs)

被引：6

作者：

Hanaki, H

Yamazaki, H

Harada, H

Kubo, R

Kobayashi, T

Atsuda, K

Sunakawa, K

机构：

[1] Zenyaku Kogyo Co Ltd, Res Lab, Nerima Ku, Tokyo 1780062, Japan

[2] Kitasato Inst, Res Ctr Antiinfect Drugs, Minato Ku, Tokyo 1088641, Japan

[3] Kanto Chem Co Inc, Dept Life Sci, Chuo Ku, Tokyo 1030023, Japan

[4] Kitasato Univ, Ctr Clin Pharm, Minato Ku, Tokyo 1088641, Japan

[5] Kitasato Univ, Clin Sci Div Hop Pharm, Minato Ku, Tokyo 1088641, Japan

[6] Kitasato Univ, Dept Infect Dis, Sagamihara, Kanagawa 2288555, Japan

来源：

JOURNAL OF ANTIBIOTICS | 2005年 / 58卷 / 01期

关键词：

ESBL; beta-lactamase; chromogenic; cephalosporin; HMRZ-86;

D O I：

10.1038/ja.2005.9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We synthesized 7-substituted-3-(2,4-dinitrostyryl)cephalosporin derivatives which were Nitrocefin analogs, for detecting extended spectrum beta-lactamase (ESBL) specifically. HMRZ-86 which has carboxypropyloxyimino group on 7-aminothiazolacetamide substituent were not hydrolyzed by class A, C and D lactamases, but it was hydrolyzed by ESBL and metallo lactamase (class B), then its color changed from yellow to red. The hydrolysis of metallo beta-lactamase was inhibited by adding sodium mercapto acetic acid (SMA). Therefore HMRZ-86 is a useful chromogenic agent to detect ESBL specifically.

引用

页码：69 / 73

页数：5

共 5 条

[1] Characterization of HMRZ-86:: a novel chromogenic cephalosporin for the detection of extended-spectrum β-lactamases [J].