Optimization of ASE conditions for the HPLC determination of rutin and isoquercitrin in Sambucus nigra L.

被引：27

作者：

Dawidowicz, AL

Wianowska, D

Gawdzik, J

Smolarz, DH

机构：

[1] Marie Curie Sklodowska Univ, Fac Chem, PL-20031 Lublin, Poland

[2] Med Univ, Dept Pharmaceut Bot, Lublin, Poland

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2003年 / 26卷 / 14期

关键词：

ASE; extraction optimization; flavonols; Sambucus nigra L;

D O I：

10.1081/JLC-120023253

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The accelerated solvent extraction (ASE) procedure has been examined as a sample preparation method for the HPLC analysis of rutin and isoquercitrin in Sambucus nigra L. The experimental extraction parameters (solvent composition, temperature, operation pressure, and static extraction time) have been optimized in order to receive the best recovery of both analytes from the flowers, leaves, and berries of black elder. The ASE recoveries obtained under the optimal extraction conditions were compared with analogous ones obtained by means of the maceration technique.

引用

页码：2381 / 2397

页数：17

共 32 条

[1] Multiresidue method for the analysis of pesticide residues in fruits and vegetables by accelerated solvent extraction and capillary gas chromatography [J].