Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces

被引：8

作者：

Brown, R ^{[1
]}

Smith, WE ^{[1
]}

Graham, D ^{[1
]}

机构：

[1] Univ Strathclyde, Dept Pure & Appl Chem, Glasgow G1 1XL, Lanark, Scotland

来源：

TETRAHEDRON LETTERS | 2001年 / 42卷 / 11期

基金：

英国生物技术与生命科学研究理事会;

关键词：

benzotriazole; HPLC; nucleic acids; phosphoramidites; solid-phase synthesis; SERS;

D O I：

10.1016/S0040-4039(01)00108-3

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A method for the addition of a benzotriazole moiety to the 5'-terminus of an oligonucleotide via phosphoramidite chemistry has been developed. Use of a monomethoxytrityl protecting group on the benzotriazole allowed fast on-column detritylation purification by reverse-phase HPLC. Surface enhanced Raman scattering (SERS) of the modified oligonucleotides was obtained from silver colloid. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：2197 / 2200

页数：4

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