Aptamers as ligands in affinity probe capillary electrophoresis

被引：241

作者：

German, I ^{[1
]}

Buchanan, DD ^{[1
]}

Kennedy, RT ^{[1
]}

机构：

[1] Univ Florida, Dept Chem, Gainesville, FL 32611 USA

来源：

ANALYTICAL CHEMISTRY | 1998年 / 70卷 / 21期

关键词：

D O I：

10.1021/ac980638h

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A DNA aptamer against IgE was labeled with fluorophore and used as a selective fluorescent tag for determining IgE by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). CE-LIF separations of samples containing fluorescently labeled aptamer and IgE were complete in less than 60 s and revealed two zones, one corresponding to free aptamer and the other to aptamer bound to IgE. The free aptamer peak decreased and bound aptamer peak increased in proportion to the amount of IgE in the sample so that IgE could be detected with a linear dynamic range of 10(5) and a detection limit of 46 pM. The assay was highly selective as aptamer was unaffected by the presence of IgG and IgE did not bind other DNA sequences. IgE was determined in serum samples with similar analytical figures of merit. Similar conditions using a thrombin aptamer allowed detection of thrombin.

引用

页码：4540 / 4545

页数：6

共 29 条

[1] SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN
BOCK, LC
GRIFFIN, LC
LATHAM, JA
VERMAAS, EH
TOOLE, JJ
[J]. NATURE, 1992, 355 (6360) : 564 - 566
[2] Use of a high affinity DNA ligand in flow cytometry
Davis, KA
Abrams, B
Lin, Y
Jayasena, SD
[J]. NUCLEIC ACIDS RESEARCH, 1996, 24 (04) : 702 - 706
[3] An enzyme-linked oligonucleotide assay
Drolet, DW
MoonMcDermott, L
Romig, TS
[J]. NATURE BIOTECHNOLOGY, 1996, 14 (08) : 1021 - 1025
[4] SELECTION INVITRO OF SINGLE-STRANDED-DNA MOLECULES THAT FOLD INTO SPECIFIC LIGAND-BINDING STRUCTURES
ELLINGTON, AD
SZOSTAK, JW
[J]. NATURE, 1992, 355 (6363) : 850 - 852
[5] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS
ELLINGTON, AD
SZOSTAK, JW
[J]. NATURE, 1990, 346 (6287) : 818 - 822
[6] ANALYSIS OF STRUCTURAL SPECIFICITY IN ANTIBODY-ANTIGEN REACTIONS BY CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION
EVANGELISTA, RA
CHEN, FTA
[J]. JOURNAL OF CHROMATOGRAPHY A, 1994, 680 (02) : 587 - 591
[7] STEREOSPECIFIC RECOGNITION OF TRYPTOPHAN AGAROSE BY INVITRO SELECTED RNA
FAMULOK, M
SZOSTAK, JW
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (10) : 3990 - 3991
[8] MOLECULAR RECOGNITION OF AMINO-ACIDS BY RNA-APTAMERS - AN L-CITRULLINE BINDING RNA MOTIF AND ITS EVOLUTION INTO AN L-ARGININE BINDER
FAMULOK, M
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (05) : 1698 - 1706
[9] DNA aptamers block L-selectin function in vivo - Inhibition of human lymphocyte trafficking in SCID mice
Hicke, BJ
Watson, SR
Koenig, A
Lynott, CK
Bargatze, RF
Chang, YF
Ringquist, S
MoonMcDermot, L
Jennings, S
Fitzwater, T
Han, HL
Varki, N
Albinana, I
Willis, MC
Varki, A
Parma, D
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1996, 98 (12) : 2688 - 2692
[10] HIGH-RESOLUTION MOLECULAR DISCRIMINATION BY RNA
JENISON, RD
GILL, SC
PARDI, A
POLISKY, B
[J]. SCIENCE, 1994, 263 (5152) : 1425 - 1429

← 1 2 3 →