Evaluation of left ventricular diastolic function by pulsed Doppler tissue imaging in mice

Background: Diastolic left ventricular (LV) function is commonly characterized by transmitral flow pattern in human beings. Recently, Doppler tissue imaging (DTI) was introduced to evaluate diastolic function. The aim of our study was to validate DTI in the evaluation of diastolic function in mice. Methods: We measured indices of diastolic function using pulsed DTI, and transmittal Doppler and LV pressure and its maximal rate of decrease (LVdP/ dt(min)), before and 4 weeks after aortic banding in C57BL/6 mice. Results: Peak early diastolic velocity and ratio of peak early-to-late filling velocities, both measured by DTI, were significantly reduced after banding, thereby indicating diastolic dysfunction. Diastolic dysfunction was confirmed by impaired LV dP/ dt(min), decreased transmitral early filling velocity, and transmitral early-to-late filling velocity ratio using transmitral Doppler. Conclusion. DTI detects diastolic dysfunction caused by chronic pressure overload in mice after aortic banding. DTI is suggested to be implemented as part of routine mouse echocardiography for evaluation of LV diastolic function.