Simple and rational approach to the identification of Mycobacterium tuberculosis, Mycobacterium avium complex species, and other commonly isolated mycobacteria

被引：32

作者：

Wong, DA

Yip, PCW

Cheung, DTL

Kam, KM

机构：

[1] Kowloon Hosp, TB Res Lab, Kowloon, Hong Kong, Peoples R China

[2] Kowloon Hosp, Publ Hlth Lab, Kowloon, Hong Kong, Peoples R China

[3] HKSAR, Dept Hlth, Pathol Serv, Hong Kong, Hong Kong, Peoples R China

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 10期

关键词：

D O I：

10.1128/JCM.39.10.3768-3771.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel PCR-restriction fragment length polymorphism analysis of the hsp65 gene was developed. The restriction patterns for Mycobacterium tuberculosis and Mycobacterium avium complex (MAC) species were designed to be highly distinct, and the overall number of restriction patterns was designed to be limited. Four hundred specimens (17 reference strains and 383 clinical isolates) were tested, of which 98 were M. tuberculosis and 132 were MAC species. The assay was virtually 100% sensitive and specific for Af. tuberculosis and MAC species. Moreover, it gave highly concordant results for other mycobacterial species other than M. terrae complex species. This assay can be completed in one day and is user-friendly and robust. Therefore, it is highly suitable for large-scale use in a clinical laboratory.

引用

页码：3768 / 3771

页数：4

共 21 条

[1] Rapid identification of mycobacteria to species level by PCR-restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species [J].