Engineering novel metalloproteins: Design of metal-binding sites into native protein scaffolds

被引：338

作者：

Lu, Y ^{[1
]}

Berry, SM ^{[1
]}

Pfister, TD ^{[1
]}

机构：

[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

来源：

CHEMICAL REVIEWS | 2001年 / 101卷 / 10期

关键词：

D O I：

10.1021/cr0000574

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The design of metal binding sites into native protein scaffolds was discussed. Most native scaffolds have high thermodynamic stability and extraordinary tolerance for residue substitution, deletion and insertion. The resulting protein can more often assume globular rather than molten globular forms and is more amenable to spectroscopic and x ray crystallographic characterization.

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页码：3047 / 3080

页数：34

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