Epidermal growth factor receptor signaling modulates chemokine (CXC) ligand 5 expression and is associated with villus angiogenesis after small bowel resection

Background. Adaptive villas growth alter a massive small bowel resection (SBR) is an important response to the loss of Intestinal surface area and is regulated via epidermal growth factor receptor (EGFR) signaling. Increased levels of the proangiogenic chemokine frond 5 (CXCL5) have been found within the adapting bowel in which angiogenesis is increased. We sought to determine whether CXCL5 was expressed specifically in the villus mesenchymal zone (area of increased blood vessel growth) and whether this expression was affected by EGF Methods. C57B1./6J mice were subjected to sham operation (bowel transaction rind, reanastomosis) or 50% proximal,SBR. The remnant intestine was harvested, and the villas lamina propria was isolated by laser capture microdissection The expression CXCL5 messenger RNA (mRNA) was analyzed using real-time polymerase chain reaction (RTPCR) Furthermore, CXGL5 mRNA levels were determined in ECF-stimulated human umbilical vein endothelial cells (HUVECs) Results. A 2 39-fold increase (P < 05) in CXCL5 mRNA occurred in the lamina propria after SBR. In addition, villas height was found to be related directly to the degree of CXCL5 mRNA (R-2 = 0.97) expression HUVEC's treated with EGF demonstrated a 9-fold increase in CXCL5 niRNA expression. Conclusion. The villas growth observed In resection-induced adaptation us associated with increased expression of the chemokine CXCL5 within the lamina propria Because EGE enhances CXCL5 expression directly in endothelial cells, EGER-directed I proangiogenic gene expression may be a critical mechanism for adaptive deal villas growth (Surgery 2010,148:364-70.)