Transfer of a Mycobacterium tuberculosis genotyping method, spoligotyping, from a reverse line-blot hybridization, membrane-based assay to the Luminex multianalyte profiling system

被引:109
作者
Cowan, LS [1 ]
Diem, L [1 ]
Brake, MC [1 ]
Crawford, JT [1 ]
机构
[1] Ctr Dis Control & Prevent, Div AIDS STD & TB Lab Res, Natl Ctr HIV STD & TB Prevent, Atlanta, GA 30333 USA
关键词
D O I
10.1128/JCM.42.1.474-477.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Spoligotyping using Luminex technology was shown to be a highly reproducible method suitable for high-throughput analysis. Spoligotyping of 48 isolates using the traditional membrane-based assay and the Luminex assay yielded concordant results for all isolates. The Luminex platform provides greater flexibility and cost effectiveness than the membrane-based assay.
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收藏
页码:474 / 477
页数:4
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