NMR spectroscopy of paramagnetic metalloproteins

被引：250

作者：

Bertini, I

Luchinat, C

Parigi, G

Pierattelli, R

机构：

[1] Univ Florence, Magnet Resonance Ctr, I-50019 Sesto Fiorentino, Italy

[2] Univ Florence, Dept Chem, I-50019 Sesto Fiorentino, Italy

[3] Univ Florence, Dept Agr Biotechnol, I-50144 Florence, Italy

来源：

CHEMBIOCHEM | 2005年 / 6卷 / 09期

关键词：

D O I：

10.1002/cbic.200500124

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This article deals with the solution structure determination of paramagnetic metalloproteins by NMR spectroscopy. These proteins were believed not to be suitable for NMR investigations for structure determination until a decade ago, but eventually novel experiments and software protocols were developed, with the aim of making the approach suitable for the goal and as user-friendly and safe as possible. In the article, we also give hints for the optimization of experiments with respect to each particular metal ion, with respect to each particular metal ion, with the aim of also providing a handy tool for non-specialists. Finally, a section is dedicated to the significant progress made on C-13 direct detection, which reduces the negative effects of paramagnetism and may constitute a new chapter in the whole field of NMR spectroscopy.

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收藏

页码：1536 / 1549

页数：14

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