A reference map of the Arabidopsis thaliana mature pollen proteome

被引:125
作者
Noir, S [1 ]
Bräutigam, A [1 ]
Colby, T [1 ]
Schmidt, J [1 ]
Panstruga, R [1 ]
机构
[1] Max Planck Inst Plant Breeding Res, D-50829 Cologne, Germany
关键词
Arabidopsis thaliana; male gametophyte; mass spectrometry; mature pollen; proteome; two-dimensional gel electrophoresis;
D O I
10.1016/j.bbrc.2005.09.185
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The male gametophyte (or pollen) plays an obligatory role during sexual reproduction of higher plants. The extremely reduced complexity of this organ renders pollen a valuable experimental system for studying fundamental aspects of plant biology Such as cell fate determination, cell-cell interactions, cell polarity, and tip-growth. Here, we present the first reference map of the mature pollen proteome of the dicotyledonous model plant species, Arabidopsis thaliana. Based on two-dimensional gel electrophoresis, matrix-assisted laser desorption/ionization time-of-flight, and electrospray quadrupole time-of-flight mass spectrometry, we reproducibly identified 121 different proteins in 145 individual spots. The presence, subcellular localization, and functional classification of the identified proteins are discussed in relation to the pollen transcriptome and the full protein complement encoded by the nuclear Arabidopsis genome. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:1257 / 1266
页数:10
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