Effects of nucleotides on cyanide-resistant respiratory activity in mitochondria isolated from antimycin A-treated yeast Hansenula anomala

被引：33

作者：

Sakajo, S

Minagawa, N

Yoshimoto, A

机构：

[1] Department of Biochemistry, Niigata College of Pharmacy, 5–13–2 Kamishin’ei-cho, Niigata

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1997年 / 61卷 / 02期

关键词：

cyanide-resistant respiration; alternative oxidase; mitochondria; nucleotides; Hansenula (Pichia) anomala;

D O I：

10.1271/bbb.61.396

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mitochondria were isolated from Hansenula anomala induced for cyanide-resistant respiration by antimycin A-treatment, Cyanide-resistant respiratory activity in isolated mitochondria was stimulated by AMP, ADP, dAMP, and GMP, but not by ATP, adenosine, cAMP, 2'(3')-AMP, CMP, and UMP. Effects of nucleotides were also observed on cyanide-resistant and salicylhydroxamate-sensitive decylubiquinol oxidase activity, Carbonylcyanide m-chlorophenylhydrazone, oligomycin, and carboxyatractyloside did not affect activation of decylubiquinol oxidase activity by AMP, It is suggested that purine nucleoside 5'-monophosphate or diphosphate stimulates alternative oxidase activity from the outer surface of the mitochondrial inner membrane with a mechanism different from respiratory control, Alternative oxidase activity might be controlled by adenine nucleotides posttranscriptionally in fungi.

引用

页码：396 / 399

页数：4

共 29 条

[1] Targeting the plant alternative oxidase protein to Schizosaccharomyces pombe mitochondria confers cyanide-insensitive respiration [J].