Interleukin-10 inhibits the down-regulation of ATP binding cassette transporter A1 by tumour necrosis factor-alpha in THP-1 macrophage-derived foam cells

被引：55

作者：

Mei, Chun-li ^{[1
]}

Chen, Zhi-jian ^{[1
]}

Liao, Yu-hua ^{[1
]}

Wang, Yan-fu ^{[1
]}

Peng, Hong-yu ^{[1
]}

Chen, Yu ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Cardiol, Wuhan 430022, Hubei Province, Peoples R China

来源：

CELL BIOLOGY INTERNATIONAL | 2007年 / 31卷 / 12期

关键词：

ATP binding cassette transporter A1; cholesterol content; tumour necrosis factor-alpha; interleukin-10; THP-1 macrophage-derived foam cells;

D O I：

10.1016/j.cellbi.2007.06.009

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

It is suggested that cholesterol efflux mediated by ATP binding cassette transporter A1 (ABCA1) plays an important role in anti-atherogenesis. However, the effects of inflammatory cytokines on ABCA1 expression and cholesterol accumulation in foam cells are little known. This study investigates the effects of tumour necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) on ABCA1 expression and cholesterol content in THP-1 macrophage-derived foam cells. ABCA1mRNA and protein levels were determined by RT-PCR and Western blot, respectively. The total cholesterol content in THP-1 macrophage-derived foam cells was detected by the zymochemistry method. Results revealed that TNF-alpha could increase cholesterol content by down-regulating ABCA1 expression in a time-dependent manner in THP-1 macrophage-derived foam cells, which may contribute to its pro-atherosclerotic effect. In addition IL-10 time-dependently decreased cholesterol accumulation by up-regulating ABCA1 expression and inhibited the down-regulation of ABCA1 by TNF-alpha in THP-1 macrophage-derived foam cells, which may be one of the mechanisms of IL-10 contributing to its anti-atherosclerotic action. (c) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

引用

页码：1456 / 1461

页数：6

共 30 条

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