In situ derivatisation using pressurized liquid extraction to determine phenols, sterols and carboxylic acids in environmental samples and microbial biomasses

被引:26
作者
Pörschmann, J
Plugge, J
Toth, R
机构
[1] UFZ Helmholtz Ctr Environm Res, Ctr Environm Res Leipzig Halle, Dept Remediat Res, D-04318 Leipzig, Germany
[2] Univ Veszprem, Inst Chem Technol, H-8201 Veszprem, Hungary
关键词
pressurized liquid extraction; extraction methods; derivatisation; GC; peat; soil; environmental analysis; phenols; sterols; carboxylic acids; fatty acids;
D O I
10.1016/S0021-9673(00)00888-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Pressurized liquid extraction was combined with in-situ derivatisation to extract polar analytes such as phenols (including chlorophenols) sterols and carboxylic acids from environmental and microbial samples. This one-step protocol uses acetic anhydride as an acetylation agent, N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) as an silylation agent, and boron trifluoride-methanol, phenyltrimethyl ammoniumhydroxide and trimethyl sulfoniumhydroxide as methylation agents. It results in faster extraction rates and better or comparable extraction efficiencies when compared to classical approaches. The addition of a silylation agent also facilitates the extraction kinetics of analytes not accessible to silylation (e.g. polycyclic aromatic hydrocarbons or alkylbenzenes). This may be attributed to a dissociative action of the agent to weaken analyte-matrix interactions. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:95 / 109
页数:15
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