DNA methylation profiling using bisulfite-based epityping of pooled genomic DNA

被引：41

作者：

Docherty, Sophia J. ^{[1
]}

Davis, Oliver S. P. ^{[1
]}

Haworth, Claire M. A. ^{[1
]}

Plomin, Robert ^{[1
]}

Mill, Jonathan ^{[1
]}

机构：

[1] Inst Psychiat, Kings Coll London, MRC Social Genet & Dev Psychiat Ctr, London SE5 8AF, England

来源：

METHODS | 2010年 / 52卷 / 03期

基金：

英国医学研究理事会;

关键词：

Epigenetics; DNA pooling; Sodium bisulfite; DNA methylation; High-throughput; ASSOCIATION SCAN; MICROARRAYS; PATTERNS; ABILITY;

D O I：

10.1016/j.ymeth.2010.06.017

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA methylation plays a vital role in normal cellular function, with aberrant methylation signatures being implicated in a growing number of human pathologies and complex human traits. Methods based on the modification of genomic DNA with sodium bisulfite are considered the 'gold-standard' for DNA methylation profiling on genomic DNA: however they require large amounts of DNA and may be prohibitively expensive when used on the large sample sizes necessary to detect small effects. DNA pooling approaches are already widely used in large-scale studies of DNA sequence and gene expression. In this paper, we describe the application of this economical DNA pooling technique to the study of DNA methylation profiles. This method generates accurate quantitative assessments of group DNA methylation averages, reducing the time, cost and amount of DNA starting material required for large-scale epigenetic investigation of disease phenotypes. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：255 / 258

页数：4

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