RFLP analysis of a PCR amplified region of chloroplast DNA in eggplant and related Solanum species

被引：40

作者：

Isshiki, S ^{[1
]}

Uchiyama, T ^{[1
]}

Tashiro, Y ^{[1
]}

Miyazaki, S ^{[1
]}

机构：

[1] Saga Univ, Fac Agr, Dept Biotechnol & Plant Breeding, Saga 8408502, Japan

来源：

EUPHYTICA | 1998年 / 102卷 / 03期

关键词：

chloroplast DNA; eggplant; PCR; RFLP; Solanum; taxonomy;

D O I：

10.1023/A:1018304308608

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

RFLP analysis of a PCR amplified 3.2-kbp region of cpDNA bounded by the conserved sequences in rbe L and ORF 106 was performed in eggplant (Solanum melongena), its related Solanum species, S. incanum, S. virginianum (= S. surattense), S. torvum, S. aethiopicum (= S. gilo), S. aethiopicum (= S. integrifolium), S. violaceum (= S. indicum), S. violaceum (= S. sanitwongsei) and S. mammosum and the reciprocal hybrids between S. aethiopicum (= S. integrifolium) and S. melongena 'Uttara'. The target region of cpDNA was amplified correctly by PCR. The amplified products were digested with each of 10 restriction enzymes (Alu I, Ase I, BamH I, Hinf I, Msp I, Rsa I, ScrF I, Sty I, Tag I and Xba I). Variations of restriction patterns among the species were recognized after digesting the amplified products with each of the seven restriction enzymes, Tag I, Alu I, Rsa I, Sty I, Ase I, Hinf I and Xba I. The restriction patterns divided the examined nine species into the following five clusters, 1) S. melongena and S. incanum, 2) S. virginianum (= S. surattense), 3) S. torvum, 4) S. aethiopicum (= S. gilo), S. aethiopicum (= S. integrifolium), S. violaceum (= S indicum) and S. violaceum (= S. sanitwongsei) and 5) S. mammosum. The restriction pattern with Alu I in each of the reciprocal hybrids between S. melongena 'Uttara' and S. aethiopicum (= S. integrifolium) was identical with that of seed parent. The present study demonstrated the availability of the PCR-RFLP analysis of cpDNA for assessing taxonomic relationships and identifying cytoplasmic parentage of interspecific hybrids in eggplant and related Solanum species.

引用

页码：295 / 299

页数：5

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