Role of glutamate-52 in the mechanism of L-lactate dehydrogenase from Bacillus stearothermophilus

被引：2

作者：

Gül-Karagüler, N ^{[1
]}

Sessions, RB ^{[1
]}

Holbrook, JJ ^{[1
]}

机构：

[1] Sch Med Sci Bristol, Dept Biochem, Bristol BS8 1TD, Avon, England

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 05期

关键词：

lactate dehydrogenase; substrate inhibition; site-directed mutagenesis;

D O I：

10.1023/A:1005687723905

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A single residue of the NAD(H)-dependent lactate dehydrogenase (LDH) from Bacillus stearothermophilus has been changed in order to decrease substrate inhibition. The conserved aspartic acid residue at position 52 was replaced by glutamate using site-directed mutagenesis. The effect on substrate inhibition was measured. In the glutamate-52 mutant substrate inhibition is decreased twofold.

引用

页码：395 / 399

页数：5

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