Genomic instability within centromeres of interspecific marsupial hybrids

被引:79
作者
Metcalfe, Cushla J. [1 ]
Bulazel, Kira V. [1 ]
Ferreri, Gianni C. [1 ]
Schroeder-Reiter, Elizabeth [3 ]
Wanner, Gerhard [3 ]
Rem, Willem [2 ]
Obergfell, Craig [1 ]
Eldridge, Mark D. B. [4 ]
O'Neill, Rachel J. [1 ]
机构
[1] Univ Connecticut, Dept Mol & Cell Biol, Storrs, CT 06269 USA
[2] Univ Cambridge, Dept Clin Vet Med, Cambridge Resource Ctr Comparat Genom, Cambridge CB2 1TN, England
[3] Univ Munich, Dept Biol 1, D-80638 Munich, Germany
[4] Australian Museum, Sydney, NSW 2010, Australia
基金
英国惠康基金;
关键词
D O I
10.1534/genetics.107.082313
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Several lines of evidence suggest that, within a lineage, particular genomic regions are subject to instability that Can lead to Specific types of chromosome rearrangements important in species incompatibility. Within family Macropodidae (kangaroos, wallabies, bettongs, and potoroos), which exhibit. recent and extensive karyotypic evolution, rearrangements involve chiefly the centromere. We propose that centromeres are the primary target for destabilization in cases of genomic instability, such as interspecific hybridization, and participate in the formation Of novel chromosome rearrangements. Here We Use standard cytological staining, cross-species chromosome painting, DNA probe analyses, and scanning electron microscopy to examine four interspecific macropodid hybrids X Macropus agilis). The parental complements share the same centric fusions relative to the persumed macropodid ancestral karyotype, but can be differentiated oil the basis of heterochromatic content,, M. rufogriseus having larger centromeres with large C-banding positive regions. All hybrids exhibited the same pattern Of chromosonal instability and remodeling specifically within the centromeres derived from the maternal (M. rufogriseus) complement. This instability included amplification of a satellite repeat and a transposable element, changes in chromatin structure, and de novo whole-arm rearrangements. We discuss possible reasons and mechanisms For the centromeric instability and remodeling observed in all four macropodid hybrids.
引用
收藏
页码:2507 / 2517
页数:11
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