Improved method for preparation of samples for the polymerase chain reaction for detection of Coxiella burnetii in milk using immunomagnetic separation

被引：16

作者：

Muramatsu, Y

Maruyama, M

Yanase, T

Ueno, H

Morita, C

机构：

[1] Dept. of Veterinary Public Health, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069

来源：

VETERINARY MICROBIOLOGY | 1996年 / 51卷 / 1-2期

关键词：

Coxiella burnetii; Cattle-Rickettsia; diagnosis Coxiella spp; immunomagnetic separation; milk; PCR;

D O I：

10.1016/0378-1135(95)00203-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Immunomagnetic separation (IMS) improved the detection of Coxiella burnetii in cow's milk by the polymerase chain reaction (PCR). IMS represents a novel approach to the isolation of microorganism from milk. The combination of IMS and PCR analysis has an adequate sensitivity for detection of C. burnetii.

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收藏

页码：179 / 185

页数：7

相关论文

共 16 条

[1] Q-FEVER - EXPERIMENTAL Q-FEVER IN CATTLE [J].

BELL, EJ ;

PARKER, RR ;

STOENNER, HG .

AMERICAN JOURNAL OF PUBLIC HEALTH, 1949, 39 (04) :478-484

[2]

CORBOZ L, 1982, EXPERIENTIA, V38, P1371

[3]

Derrick E. H., 1942, AUSTRALIAN JOUR EXP BIOL AND MED SCI, V20, P105, DOI 10.1038/icb.1942.19

[4] DIAGNOSIS OF MEDITERRANEAN SPOTTED-FEVER BY INDIRECT IMMUNOFLUORESCENCE OF RICKETTSIA-CONORII IN CIRCULATING ENDOTHELIAL-CELLS ISOLATED WITH MONOCLONAL-ANTIBODY COATED IMMUNOMAGNETIC BEADS [J].

DRANCOURT, M ;

GEORGE, F ;

BROUQUI, P ;

SAMPOL, J ;

RAOULT, D .

JOURNAL OF INFECTIOUS DISEASES, 1992, 166 (03) :660-663

[5] EXCESS ANTIBODY IMMUNOASSAY FOR RAT GLANDULAR KALLIKREIN - MONOSIZED POLYMER PARTICLES AS THE PREFERRED SOLID-PHASE MATERIAL [J].

JOHANSEN, L ;

NUSTAD, K ;

ORSTAVIK, TB ;

UGELSTAD, J ;

BERGE, A ;

ELLINGSEN, T .

JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 59 (02) :255-264

[6] STAPHYLOCOCCUS-AUREUS EXOPOLYSACCHARIDE INVIVO DEMONSTRATED BY IMMUNOMAGNETIC SEPARATION AND ELECTRON-MICROSCOPY [J].

JOHNE, B ;

JARP, J ;

HAAHEIM, LR .

JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (07) :1631-1635

[7]

MCCAUL TF, 1991, ACTA VIROL, V35, P545

[8] APPLICATION OF SOLID-PHASE ANTIBODIES TO RADIOIMMUNOASSAY - EVALUATION OF 2 POLYMERIC MICROPARTICLES, DYNOSPHERES AND NYLON, ACTIVATED BY CARBONYLDIIMIDAZOLE OR TRESYL CHLORIDE [J].

MCCONWAY, MG ;

CHAPMAN, RS .

JOURNAL OF IMMUNOLOGICAL METHODS, 1986, 95 (02) :259-266

[9]

REED L. J., 1938, AMER JOUR HYG, V27, P493

[10]

Sambrook J., 1989, MOL CLONING LABORATO, V2nd